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Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (33): 148-158.doi: 10.11924/j.issn.1000-6850.casb17070096

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Technique Optimization for Extracting Total Flavonoids in Anoectochilus Roxburghii by Ultrasonic-Assisted Solvent

  

  • Received:2017-07-19 Revised:2017-08-15 Accepted:2017-08-24 Online:2017-11-27 Published:2017-11-27

Abstract: This paper aims at studying the optimum extraction condition of total flavonoids in Anoectochilus roxburghii. By taking ultrasonic-assisted extraction technique, based on the single factor test, the extraction conditions of total flavonoids in Anoectochilus roxburghii were optimized by response surface methodology. The results showed that the extraction rate of total flavonoids in Anoectochilus roxburghii was 2.13% at the condition of Anoectochilus roxburghii grain size of 100 mesh, the alcohol volume fraction of 40%, the ratio of solid to liquid of 1:40 (mg:mL), ultrasonic power of 250 W, ultrasonic time of 30 min. The extraction effect of total flavonoids in Anoectochilus roxburghii was strengthened by using ultrasonic-assisted extraction technique.

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