Abstract: To provide guidance for barley utilization in Hunan Province,‘Huadamai 9’from Hubei Province was used as the research object in this study. By measuring the plant height, tiller number, fresh weight and forage quality, the effects of different mowing periods and stubble heights on yield and quality of fresh barley were analyzed and the appropriate mowing period and stubble height were screened out. The results showed that the growth period of‘Huadamai 9’was 169 days. The highest plant height of‘Huadamai 9’was at initial heading stage and the tiller number ranged from 3-5. It was used for forage by harvesting the whole plant of barley in Dongting Lake area. The mowing period could be started from the initial heading stage, and the full heading stage was the best. The stubble height could be 5-10 cm, and 5 cm was the optimum for higher yield. For‘Huadamai 9’, the optimal stubble height was 5 cm, mowing period was at full heading stage, and the total yield reached 42.23 kg/13.5 m2. There were significant differences in the contents of crude ash, crude protein and crude fiber in different periods. The content of crude protein was not significantly different between initial heading stage and full heading stage, but it was the highest at initial heading stage. The crude fiber content was the highest at maturation stage, while the content of crude ash was not significantly different at different cutting time.