Amino Acid Sequence Analysis of 7s Globulin (β-conglycinin) of Soybean

doi:10.11924/j.issn.1000-6850.casb17070111

Chinese Agricultural Science Bulletin ›› 2018, Vol. 34 ›› Issue (26): 48-51.doi: 10.11924/j.issn.1000-6850.casb17070111

Special Issue: 生物技术；油料作物

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Amino Acid Sequence Analysis of 7s Globulin (β-conglycinin) of Soybean

杨青春, and 张保亮

Received:2017-07-21 Revised:2017-09-12 Accepted:2017-09-21 Online:2018-09-19 Published:2018-09-19

Abstract

Abstract: To study the effect of amino acid composition of different subunits of 7s globulin (β -conglycinin) on the nutritional quality of soybean, the amino acid sequence compositions and comparison of 7s globulin (β -conglycinin) subunit of soybean submitted to GenBank database were studied by means of bioinformatics software (BIOEDIT and DNAMAN). The results showed that the contents of tryptophan, methionine and cystine in the three subunits were lower, and theβ- subunit was free of tryptophan. The three subunits were rich in glutamic acid and leucine, the content of glutamic acid was the highest inα -subunit, which was 13.16%, and the content of leucine in β- subunit was the highest, which was 10.48% . Glutamine, arginine and serine content were also relatively high. Amino acid sequence alignment analysis showed that there were more conserved sequences, and the homologous tree showed that the genetic relationship among the three subunits was close.

杨青春, and 张保亮. Amino Acid Sequence Analysis of 7s Globulin (β-conglycinin) of Soybean[J]. Chinese Agricultural Science Bulletin, 2018, 34(26): 48-51.

References

[1] 王连铮,王金陵.大豆遗传育种[M]. 北京:科学出版社,1992,260.
[2] 杨春燕,姚利波,刘兵强,等.国内外大豆品质育种研究方法与最新进展[J].华北农学报,2009,24(增刊):75-78.
[3] 姜振峰,赫卫,汪洋,等.大豆种子7S、11S 球蛋白及7S 球蛋白亚基的研究[J].中国油料作物学报,2007,29(2):32-35.
[4] I. C. Peng, D. W. Quass, W. R. Dayton, et al.The physicochemical and functional properties of soybean 11S globulin-a review[J].Cereal Chemistry,1984,61(6):480-489.
[5] Miroljub B. Barac,Sladana P. Stanojevic,Snezana T. Jovanovic,et al.SOY PROTEIN MODIFICATION - A REVIEW[J].APTEFF,2004,35:3-16.
[6] Meinke D W,Chen J,Beachy R N.Expression of storage protein genes during soybean seed development[J].Planta,1981,153:130-139.
[7] Fukushina D.Recent progress of soybean protein foods[J].Food Rev Int,1991,7(3):323-351.
[8] 黄丽华,麻浩,王显生,等.大豆种子贮藏蛋白11S 和7S 组分研究[J].中国油料作物学报,2003,25(3):20-23.
[9] 裴东红,余建章,程国华,等.不同大豆品种豆腐产量的研究[J].大豆科学,1995,14(1):13-20.
[10] 关荣霞,常汝镇,邱丽娟,等.栽培大豆蛋白亚基11S/7S 组成及过敏蛋白缺失分析[J].作物学报,2014,30(11):1076-1079.
[11] Ping X,Ha as E J,Zeece M.G,et alC-Terminal 23kDa polypeptide of soybean Gly m Bd 28 K is a potential allergen[J].Planta,2004,220:56-63.
[12] 孟岩,郭顺堂,邱丽娟,等.蛋白质亚基缺失的特异大豆的筛选及β亚基在大豆加工中的作用[J].食品工业科技,2003,(z1):28-31.
[13] 刘珊珊,孟凡立,刁桂珠,等.大豆7S 球蛋白(α+β)-亚基缺失型种质的α-与β-亚基基因的测序与分析[J].中国农业科学,2009,42(2):419-424.
[14] Hajika M M,Takahashi S Sakai,Igita M. Anew genotype of 7S globulin(β-conglycinin) detected in wild soybean (Glycine soja Sieb.et Zucc)[J].Breed Sci,1996,46:385-386.
[15] 田　颖,时明慧.赖氨酸生理功能的研究进展[J].美食研究,2014,31(1):60-64.
[16] 刘珊珊,田福东,高丽辉,等.大豆7S 球蛋白亚基组成对品质性状的影响[J].作物学报,2008,34(5):909-913.
[17] Coates J B,Medeiros J S,Thanh V H.Characterization of the subunits of β-conglycinin[J].ArchBiochem Biophys,1985,243:184-194.
[18] Staswick PE,Nielsen NC.Characterization of a soybean cultivar lacking certain glycinin subunits[J].Arch Biochem Biophys,1983,223(1):1-8.
[19] Ladin BF,Doyle JJ,Beachy RN.Molecular characterization of a deletion mutation affecting the alpha’-subunit of beta-conglycinin of soybean[J].J Mol Appl Genet,1984,2(4):372-380.
[20] 宋波,蓝岚,田福东,等.大豆7S球蛋白α'亚基缺失及(α'+α)亚基双缺失品系的回交转育[J].作物学报,2012S,S38S(12) :2297-2305.

Amino Acid Sequence Analysis of 7s Globulin (β-conglycinin) of Soybean

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