Abstract: To rectify the excessive application of phosphate fertilizer and achieve the goal of zero-growth of fertilizer, this study was conducted to evaluate the effects of phosphorus fertilizer reduction on yield and quality of greenhouse tomato and soil available phosphorus under different conditions, using a field test method. There were five treatments including conventional phosphorus fertilization, 30% reduction of phosphorus fertilizer, 60% reduction of phosphorus fertilizer, 100% reduction of phosphorus fertilizer and no fertilizer-applying. The results showed that soil available phosphorus content and the potassium content of plant were the highest under the conventional fertilizer condition. The N content of plant and fruit, the yield and the soluble solid content were the highest under the 30% phosphorus fertilizer reduction; the P content of plant and the K content of fruit were the highest under the 60% phosphorus fertilizer reduction; the Vc content of tomato was the highest under the 100% phosphorus fertilizer reduction. Therefore, the yield and quality of tomato were not decreased but slightly improved under suitable phosphorus fertilizer reduction.