Effects of Resveratrol on Growth Performance, Immunity and Liver Microstructure of GIFT (Oreochromis niloticus)

doi:10.11924/j.issn.1000-6850.casb17090040

Abstract

Abstract: Resveratrol has gained great interest due to various reported health benefits. However, little information is available about its effects on the genetically improved farmed tilapia (GIFT), Oreochromis niloticus. Hence, this study was conducted to investigate the effects of dietary supplemental resveratrol on the growth, non-specific immunity, intestinal villi and liver microstructure of GIFT. (Method) Fish were fed four diets with different levels of resveratrol: 0, 250, 500 and 1000mg/kg. Eight non-specific immune enzymes in serum were measured on the 45th day. (Result)The results showed that the weight gain rate (WGR), specific growth rate (SGR) and feed coefficient (FC) significantly decreased (P<0.05) with increment of resveratrol at average 6.5%, 4.0% and 8.3% respectively. Activities of C3, C4, IgM, TNF-α, Hsp-60 and TRF decreased with increasing addition of resveratrol. Furthermore, differentSdegrees of damage were observedSin intestinal villi and liver in treated groups. (Conclusion)Therefore, addition of 0.1% resveratrol in diets can partially decrease non-specific immune enzyme activities, and damage the intestine and liver of tilapia.

References

[1] Park EJ. The pharmacology of resveratrol in animals and humans[J]. Biochimica Et Biophysica Acta, 2015,1852 (6):1071-1113.
[2] Magyar K, Halmosi R, Palfi A. Cardioprotection by resveratrol: A human clinical trial in patients with stable coronary artery disease[J]. Clinical hemorheology and microcirculation,2012,50(3):179-187.
[3] Bo S, Ciccone G, Gastiglione A, et al. Anti-inflammatory and antioxidant effects of resveratrol in healthy smokers a randomized, double-blind, placedo-controlled, cross-over trial[J]. Curr Med Chem,2013,20(10):1323-1331.
[4] 李先宽,李赫宇,李帅,等.白藜芦醇研究进展[J].中草药,2016,47(14):2568-2578.
[5] 胡红莲,高民.肠道屏障功能及其评价指标的研究进展[J].中国畜牧杂志,2012,48(17):78-82.
[6] 董婧,王洋,郭锦晓,等.白藜芦醇强化卤虫对斑马鱼肠道的形态结构和抗氧化能力的影响[J].沈阳农业大学学报,2016,47(04):488-492.
[7] 黄超.水体铜和锌暴露对矛尾复虾虎鱼肝脏脂代谢的影响[D].武汉:华中农业大学,2015,14-15.
[8] 崔培,范泽,李建,等.饲料中添加白藜芦醇对血鹦鹉肝脏生化指标的影响[J].大连海洋大学学报,2015,(2):203-206.
[9] 闫亚楠,夏斯蕾,田红艳,等.白藜芦醇对高脂胁迫团头鲂抗氧化能力、非特异免疫机能和抗病力的影响[J].水生生物学报,2017,41(01):155-164.
[10] 贺艳辉,张红燕,龚赟翀,等.我国罗非鱼养殖品种及养殖发展分析[J].水产养殖,2009,30(2):12-14.
[11] 王爱民,杨文平,於叶兵,等.不同脂肪含量饲料对吉富罗非鱼鱼种生长性能、脂蛋白脂酶活性及其基因表达的影响[J/OL]. http://auth.njau.edu.cn:2360/kcms/detail/j.cnki.hnlkxb.2014.02.011.html,2017-08-28.
[12] 孙盛明,谢骏,朱健,等.饲料中添加甘露寡糖对团头鲂幼鱼生长性能、抗氧化能力和肠道菌群的影响[J].动物营养学报,2014,(11):3371-3379.
[13] 张金花,王树迎.泰山螭霖鱼肠道的显微和超微结构[J].中国兽医学报,2004,24(2):163-167.
[14] 黄布敏.虹鳟Oncorhynchus mykis肝脏形态学研究及“富康163”对肝脏结构的影响[D].兰州:甘肃农业大学,2012,13-14.
[15] 武斌,李宗芸,杨素春,等.几种热激蛋白在细胞凋亡信号通路中的调控作用[J].中国生物化学与分子生物学报,2011,27(1):22-31.
[16] Hana S, Choia J R, Shinb K, et al. Resveratrol up regulated heat shock proteins and extended the survival of G93A-SOD1 mice[J]. Brain research,2012 ,1483(05):112-117.
[17] 张静,詹勇,苏彬.鱼类补体系统的研究进展[J].中国饲料,2008,01(05):8-11.
[18] 沈路路.虎杖的抗补体活性成分研究[D].上海:复旦大学,2013,41-42.
[19] 黄璟,周毅.肿瘤坏死因子α及其抑制剂的研究进展[J].国际口腔医学杂志,2015,42(1):63-68.
[20] 杜金梁,曹丽萍,刘英娟,等.甘草提取物对2,3,7,8-四氯二苯并-p-二噁英致建鲤精密肝切片组织损伤中生化指标及免疫功能的影响[J].农业环境科学学报,2015,34(5):837-843.
[21] Jiyun Ahn. Resveratrol inhibits TNF-α-induced changes of adipokines in 3T3-L1 adipocytes[J].Biochemical Biophysical Research Communications,2007,364(4):972-7.
[22] Liu C W, Sung H C, Lin S R, et al. Resveratrol attenuates ICAM-1 expression and monocyte adhesiveness to TNF-α-treated endothelial cells: evidence for an anti-inflammatory cascade mediated by the miR-221/222/AMPK/p38/NF-κB pathway[J/OL]. Scientific Reports,2017, doi: 10.1038/srep44689.
[23] Villar M, Ortiz G, Abundis M, et al. Effect of resveratrol administration on metabolic syndrome, insulin sensitivity, and insulin secretion[J]. Metab Syndr Relat Disord,2014,235(02):497-501.
[24] 楼允东.组织胚胎学[M].北京:中国农业出版社,2000:101- 104.
[25] 黄超.白藜芦醇对氧化应激肉鸡肠道结构和抗氧化能力的影响[C]//畜牧与动物医学:中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集.武汉:中国畜牧兽医学会动物营养学分会,2016:313.
[26] T?r?k NJ. Recent advances in the pathogenesis and diagnosis of liver fibrosis[J]. Journal of gastroenterology,2008,43(5):315-321.
[27] 冯文强.SIRT1在小鼠肝纤维化中的作用及其相关分子的组学研究[D].西安:第四军医大学,2015,34-35.
[28] 李扬扬,张启瑜,朱千东,等.白藜芦醇调节肝星状细胞活性的实验研究[J].肝胆胰外科杂志,2013,25(4):303-306.
[29] Yuki M, Hideyuki H, Kou S, et al. Low dose resveratrol ameliorates mitochondrial respiratory dysfunction and enhances cellular reprogramming[J]. Mitochondrion, 2017,34(01):43-48.
[30] 王英慧.成纤维细胞的生物学特性及其成骨作用的研究进展[J].大同医学专科学校学报,2006,26(2):30-32.