Abstract: To establish HPLC fingerprints of herbs of Rongshu oral liquid and HPLC detecting method of four composition herbs of the liquid, fingerprints of herbs (Ziziphi Spinosae Semen, Angelica sinensis radix, Schisandra chinensis and Rosae Rugosae Flos) were investigated by HPLC. With quercetin and kaempferol as indexes, the HPLC method was established to determine the quality of Rongshu oral liquid finished product. from different regions could be significantly distinguished. In addition, the HPLC method was successfully established to detect the content of quercetin and kaempferol in Rongshu oral liquid, for quercetin (5.79-38.6 μg/mL, r =0.9999) and kaempferol (3.68- 24.55 μg/mL, r =0.9999), and the average recovery value was 100.89% and 99.86%, respectively, withRSD as 1.96% (n =6) and 1.39% (n =6), respectively. The established method was accurate, stable and suitable for the quality control of Rongshu oral liquid.