Abstract: The purpose of this study is to induce adventitious buds directly from young shoots rather than callus, and establish the regeneration system of Hemerocallis fulva. The young shoots of Hemerocallis fulva‘32- 1’were used as explants, the culture medium with different hormones and activated carbon was prepared. The effects of every factor on plant regeneration of Hemerocallis fulva were studied. The results showed that different matching of hormones had significant difference on primary culture, multiplication and rooting culture of Hemerocallis fulva‘32-1’. The optimum initiation medium was MS+6-BA 3.0 mg/L+NAA 2.0 mg/L, the initiation rate and proliferation coefficient was 100.00% and 5.60, respectively, and this medium could induce the adventitious buds directly. The optimal multiplication medium was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L, and the multiplication coefficient was 3.47. The appropriate rooting medium was 1/2MS+NAA 1 mg/L+AC 2 g/L.