Abstract: Capillary electrophoresis (CE) has been widely used in the field of pesticide residues after several decades of continuous development and improvement. However, capillary electrophoresis detection of organic heterocyclic herbicides has not yet been studied. In this paper, CE determination methods of five organic heterocyclic herbicides (Simazine, Cyanazine, Simetryn, Prometryn and Terbutylazine) were established by screening CE detection parameters, 10 mmol/L borax was used as the running buffer, pH 9.5, 30 mmol/L sodium dodecyl sulfate and 7.5% methanol solution were used as the best separation medium; other conditions were injection pressure of 0.5 psi, injection time of 5 s, separation voltage of 15 kV, capillary column temperature of 25℃, detection wavelength of 221 nm. The efficient, fast and accurate detection of five kinds of organic heterocyclic herbicides could be achieved in one-time.