Abstract: To investigate the effect of different ratios of nutrients on growth and development and photosynthetic characteristics of tobacco under the condition of drip irrigation, the water and fertilizer experiment that used‘NC55’as experiment material was conducted, to compare and analyze plant height, leaf area, photosynthetic rate, and chlorophyll under six different ratios of nutrients. The results showed that by topdressing high nitrogen water soluble fertilizer in tobacco field 28 days after transplanting (N:P:K = 30:10:10), top- dressing water soluble fertilizer 35 days after transplanting (N:P:K=15:15:30), and top- dressing high potassium water soluble fertilizer 42 days after transplanting (N:P:K=12:8:38), or top- dressing potassium nitrate 28, 35, and 42 days after transplanting, better leaf area and plant height could be obtained, as well as more accumulated dry matter, and high net photosynthetic rate in vigorous growing stage. At the same time, the treatment could also get higher total chlorophyll and carotenoid content in earlier growing stage, and had obvious degradation of chlorophyll and carotenoid in late growth stage, which is good for harvest.