Abstract: This study aims to explore the diversity of different functional leaves of rice under different nitrogen fertilizer treatments, and provide a theoretical basis for selecting breeding materials with large genetic variation and large differences of agronomic traits. In this experiment, 52 rice varieties from rice germplasm resources were used as materials, and normal nitrogen and non-nitrogen treatments were set up to analyze the characters of functional leaves. The results showed that there was abundant genetic diversity of morphological characteristics of functional leaves under different nitrogen fertilizer treatments. Under different treatments, the length of the 1st functional leaf had significantly or extremely significant-positive correlation with the 2nd functional leaf and the 3rd functional leaf. The width of the 1st functional leaf had extremely significantpositive correlation with the 2nd functional leaf and the 3rd functional leaf. After principal component analysis, three principal components were obtained under normal nitrogen condition and non-nitrogen condition, the cumulative contribution rate was 94.5% and 90.6%, respectively. Through the comprehensive evaluation of every materials, the top five rice materials were‘Diantun 502’,‘Yumidao’,‘Yahui 2115’,‘Gumei 3’and This study aims to explore the diversity of different functional leaves of rice under different nitrogen fertilizer treatments, and provide a theoretical basis for selecting breeding materials with large genetic variation and large differences of agronomic traits. In this experiment, 52 rice varieties from rice germplasm resources were used as materials, and normal nitrogen and non-nitrogen treatments were set up to analyze the characters of functional leaves. The results showed that there was abundant genetic diversity of morphological characteristics of functional leaves under different nitrogen fertilizer treatments. Under different treatments, the length of the 1st functional leaf had significantly or extremely significant-positive correlation with the 2nd functional leaf and the 3rd functional leaf. The width of the 1st functional leaf had extremely significantpositive correlation with the 2nd functional leaf and the 3rd functional leaf. After principal component analysis, three principal components were obtained under normal nitrogen condition and non-nitrogen condition, the cumulative contribution rate was 94.5% and 90.6%, respectively. Through the comprehensive evaluation of every materials, the top five rice materials were‘Diantun 502’,‘Yumidao’,‘Yahui 2115’,‘Gumei 3’and ‘Shuiyuan 258’under the normal nitrogen condition, and the top five rice materials were‘Yumidao’, ‘Diantun 502’,‘Baxidao’,‘CDR22’and‘Gumei 3’under the non-nitrogen condition. In rice plant type breeding, we should use germplasm resources with abundant genetic diversity and select germplasm materials with larger genetic distance and lager variation of comprehensive traits.