Abstract: To undersdand genetics mechanism of high frequence transfer of monosomic addition line M14, charecters of monosomic addition lines and aerial axperiment materials of beet were analyzed by means of ovule separation, pollen abortion investigation and sugar content detection method. The results showed that the facultative apomixis phenomenon was exist in monosomic addition line M14. The ratio of apomixis was 96.5%, and pollen abortion was above 90%. Blastocyst was formed through megasporocyte mitosis and sperm-egg binding was not needed for breed offspring. The ratio of zoogamy was 3.5% and sexual reproduction plants maybe exist a lethal gene. The results also showed that sugar contents of monosomic addition lines and aerial axperiment materials were higher than that of cultivated beets. The exclusive genetics charecters of distant hybridization and aerial axperiment materials of sugar beet were determined by the beta corolliflora chromosome carried with high sugar content, disease resistance as well as apomixis genes. These results will be useful for exploiting beta corolliflora beneficial genes on chromosome engineering.