Abstract: This study aims to select sugar beet varieties with high resistance to Cercospora leaf spot (CLS) and high sugar content, thus to improve the economic benefits. From 2015 to 2016, we conducted field experiments with natural incidence analysis, 15 varieties were used as treatments with randomized block arrangement and four repeats. CLS index and sugar content in commercial sugar beet varieties were investigated. Statistical analysis results showed that the resistance to CLS among different sugar beet varieties showed extremely significant difference, the resistance to CLS was not stable, and the difference among different years was relatively large. The sugar contents among different sugar beet varieties showed extremely significant difference. The sugar contents of the same variety in different years had difference. In conclusion, the sugar beet variety with high and stable CLS resistance and high sugar content is‘KWS1197’, the average disease index and sugar content of two years are 18.34 and 14.01%, respectively.