Investigation of White Spot Syndrome Virus in Different Procambarus clarkii Populations in Hubei

doi:10.11924/j.issn.1000-6850.casb17120043

Abstract

Abstract: To obtain the white spot syndrome virus (WSSV) carrying situation in Procambarus clarkii, PCR detection on WSSV was conducted on 741 samples of Procambarus clarkii from 7 aquaculture areas in Hubei from May 2016 to July 2017. 49 positive PCR products were sequenced and analyzed. The results showed that over 65% of the samples carried WSSV from areas of Qianjiang City, Jianli County and Xindi in Honghu City, and about 40% of the samples carried WSSV from areas of Ezhou City, Suizhou City and Luoshan Town in Honghu City. The sequencing results indicated that A/T base mutation was found in the conserved region of WSSV of some samples. The phylogenetic tree analysis displayed that there was a farther genetic relationship between the WSSV samples with A/T base mutation and 17 WSSV subspecies in GenBank. However, a closer genetic relationship was found between the WSSV samples without A/T base mutation and the strains from Australia (MF161441) and Bangladesh (KJ719075). This study could provide a reference for the prevention and control of WSSV in Procambarus clarkii.

CLC Number:

S945.4

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