Abstract: The solid-fermentation conditions of imidacloprid-degrading strain and its suitable solid-fermentation medium composition were optimized and determined in this paper, with the index of the biomass. Effects of the fermentation conditions, such as medium component, inoculation, fermentation time and temperature, were discussed by a single factor experiment, and the optimal fermentation condition of solid fermentation was determined by using response surface analysis, and then the remediation effects of microbial agents on imidaclorprid on the tea leaf were also studied. The results showed that the optimal fermentation conditions were as follows: plant ash 65%, corn pink 8%, bran 24%, glucose 2.3%, KH2PO3 0.5%, MgSO4 0.2%; fermentation time 72.8h, inoculum amount 5.04%, fermentation temperature 28.89℃. Under these conditions, the number of biomass researched 9.537×108 cfu/g. After 12 days, degradation rate of imidacloprid on the tea leaf reached 82.0%, showing the extent imidacloprid remediation potential.