Abstract: In order to provide a scientific basis for the Huainan red wheat of strong gluten wheat production in Jiangsu province,by exploring the effects of plant density and nitrogen fertilizer on yield and economic efficiency of red strong gluten wheat. The red high yield and quality of strong gluten wheat cultivar Zhenmai 12 was used as tested material,which was bred by Zhenjiang institute of agricultural science in the hilly area of Jiangsu province , using randomized block design, with two factors of density and nitrogen The experiments were conducted to study the effect of plant density (225×10₄ ,300×10₄and 375×10₄ seedlings/hm₂) and nitrogen application rate(90,120 and 150 kg/hm₂) on the grain yield and economic efficiency of Zhenmai 12. The results showed that the influence of plant density,nitrogen application and interaction effect on grain yield was significant or highly significant, and the influence of plant density and the interaction between plant density and nitrogen application on economic efficiency of Zhenmai 12 was highly significant.Under the experimental conditions, the combination treatment of M1N3 had the highest grain yield and economic efficiency with the spike number of 435.6×10₄ spike/hm₂, 37.1 grains per panicle,45.3g per 1000 grains. The grain yield and economic efficiency were 6519.8 kg/hm₂ and 4438.7 yuan/hm₂ respectively.