Abstract: To screen effective drugs for controlling tobacco Bemisia tabaci, toxicity test and field efficacy of 3 biological insecticides to B.tabaci were used on tobacco. The toxicity test results showed that the virulence of B.tabaci presented as 0.5% ivermectin EC > 0.5% veratridine Solution > 6% rotenone microemulsion, and the LC50 values of them were 1.396 mg/L, 5.083 mg/L, 12.287 mg/L respectively, which was significantly better than the CK cyhalothrin (LC50 = 30.67 mg/L). Results of field efficacy showed that the adjusted efficiency of 3,7,14 days after treatment of 2000 times of dilution of 6% rotenone microemulsion, 0.5% ivermectin EC, 0.5% veratridine solution were over 70%. Therefore, the 3 biological insecticides have no harmful effects on the tobacco field, and the tobacco plants are safe to grow and can be further promoted and applied in production.