Biological Fertilizer: Effects on Enzyme Activity and Microbial Community Structure in Rice Soil

doi:10.11924/j.issn.1000-6850.casb18040010

Abstract

Abstract: To explore the responses of soil enzyme activities and microbial community structure in rice soil to biological fertilizer (QD) application, 3,5-dinitrosalicylic acid colorimetric method, phenylbiphenyl phosphate colorimetric method, sodium phenol-sodium hypochlorite colorimetric method and potassium permanganate titration were used to determine the activities of sucrase, phosphatase, urease and catalase in the soil. The soil genome was sequenced based on the Illumina HiSeq sequencing platform. The results showed that compared with CK, QD application significantly enhanced the activities of urease and phosphatase in soil (P<0.05), but the activities of catalase and invertase did not change significantly (P>0.05). After soil genome sequencing, Proteobacteria and Ascomycota were the dominant phylum in bacteria and fungi, respectively. The α-diversity of fungi significantly decreased after QD application, yet the α- diversity of bacteria did not change significantly. To a certain extent, the results provide a theoretical basis for the use of biological fertilizers and field soil management.

References

[1]付小猛, 毛加梅, 沈正松, 等. 中国生物有机肥的发展现状与趋势[J].湖北农业科学,2017,56(03):401-404.
[2]苏建党. 探讨过量施用化肥的危害及应对措施[J]. 农业与技术, 2016, 36(06): 49.
[3] Xia G-L, Bi J, Zhang P, et al. Effect of a new bio-organic fertilizer ontomato growth and soil activity quality. Chinese Journal of Soil Science , 2007, 38( 3) : 519－522.
[4]Ni Z-H, Ma G-R. Effect of bioactivated organo-inorgano-mixed fertilizer on growth of cabbage and soil biological activity. Chinese Journal of Soil Science, 2002, 33 ( 3) : 212 － 215 .
[5]Han X-L, Zhang N-W, Jia J-F. Effects of biological organic-inorganic compound fertilizer on yield，quality of tomato and soil. Soils and Fertilizers, 2005 ( 3) : 50－53.
[6]孙建, 刘苗, 李立军, 等. 不同施肥处理对土壤理化性质的影响[J]. 华北农学报, 2010, 25(04): 221-225.
[7] Vinale F, Marra R, Lorito M, et al. Trichoderma-plant pathogen interactions [J]. Soil Biology and Biochemistry, 2008, 40(2):1-10.
[8]李松鹏, 崔琳琳, 程家森, 等. 两株哈茨木霉菌株防治水稻纹枯病及促进水稻生长的潜力研究[J/OL]. 植物病理学报: 1-14[2018-01-24].
[9]徐文, 黄媛媛, 黄亚丽, 等. 木霉-植物互作机制的研究进展[J]. 中国生物防治学报, 2017,33(03):408-414.
[10]李世贵, 吕天晓, 顾金刚, 等. 土壤微生物分子生态学研究方法[J]. 中国土壤与肥料, 2008(06):1-4+14.
[11]关松萌. 土壤酶及其研究[M]. 北京: 农业出版社, 1986: 255-258.
[12]张智猛, 慈敦伟, 张冠初, 等. 山东地区盐碱土花生种子际土壤微生物群落结构的研究[J]. 微生物学报, 2017, 57(4):582-596.
[13]Degnan P H, Ochman H. Illumina-based analysis of microbial community diversity[J]. The ISME Journal, 2012, 6(1):183-194.
[14]CAPORASO J G, LAUBER C L, WALTERS W A, et al. Ulter-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms[J]. The ISME Journal 2012, 6(8):1621-1624.
[15]Caporaso J G, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods, 2010, 7(5):335-336
[16]Edgar R C, Haas B J, Clemente J C, et al. UCHIIME improves sensitivity and speed of chimera detection[J].Bioinformatics, 2011, 27(16):2194-2200
[17]Edgar RC. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nature Methods,2013, 10(10):996-998
[18]Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J]. Nucleic Acids Research, 2013, 41: 590-596
[19]关松荫, 郑洪元. 土壤酶及其研究法[M ] 北京: 中国农业出版社, 19 8 6.
[20]Gu J, Li S-X, Qin Q-J, et al. Changes of some hydrolase activities during agricultural waste materials composting in high temperature and static state. Chinese Agricultural Science Bulletin, 2005, 21( 5) : 32-35 .
[21]桓明辉, 李杨, 刘晓辉,等.秸秆生物菌肥对保护地土壤微生物和土壤酶活性的影响[J]. 山东农业科学, 2013, 45(01):107-109+116.
[22]Marcote I, Hernández T, Garcíndez T, et al. Influence of one or two successive annual applications of organic fertilizers on the enzyme activities of a soil under barley cultivation. Bioresource Technology, 2001, 79: 147 －154
[23]荆瑞勇, 王丽艳, 郭永霞. 生物有机肥对盆栽小白菜土壤酶活性和微生物数量的影响[J].水土保持研究, 2015, 22(02): 79-83+89.
[24]宋以玲, 于建, 肖承泽, 等. 生物菌水溶肥对土壤微生物、土壤酶活性及萝卜产量和品质的影响[J]. 山东农业科学, 2017, 49(11):74-81.
[25]Shukla SK, Yadav RL, Suman A, et al. Improving rhizospheric environment and sugarcane ratoon yield through bio-agents amended farm yard manure in udic ustochrept soil [J]. Soil and Tillage Research, 2008, 99: 158-168.
[26]Vo?í?ková J, Baldrian P. Fungal community on decomposing leaf litter undergoes rapid successional changes [J]. The ISME Journal, 2013, 7: 477-486.
[27]张风革, 霍云倩, 孙艺, 等. 连续施用生物有机肥对草地生物量及土壤微生物区系的影响[J/OL]. 南京农业大学学报, 2018(02):1-11.
[28]Chen L H, Huang X Q, Zhang F G, et al. Application of Trichoderma harzianum SQR-T037 bio-organic fertiliser significantly controls Fusarium wilt and affects the microbial communities of continuously cropped soil of cucumber [J]. Journal of the Science of Food and Agriculture, 2012, 92:2465-2470.
[29]Luo J, Ran W, Hu J, et al. Application of bio-organic fertilizer significantly affected fungal diversity of soils [J]. Soil Science Society of American Journal, 2010, 74 (6): 2039-2048.
[30]Zhang FG, Zhu Z, Yang XM, et al. Trichoderma harzianum T-E5 significantly affects cucumber root exudates and fungal community in the cucumber rhizosphere [J]. Applied Soil Ecology, 2013, 72: 41-48.
[31]张量, 张敬泽. 渐绿木霉抑菌物质的分离纯化及其对植物病原菌的抑制作用[J]. 中国农业科学, 2015, 48(05):882-888.
[32]朱兆香, 庄文颖. 木霉属研究概况[J]. 菌物学报, 2014, 33(06):1136-1153.
[33]王麟, 谭春明, 于刚, 等. 嗜杀酵母的生物学特性及其应用[J]. 天津农学院学报, 2017, 24(01):83-87+92.
[34]王祥红, 贾仁洁, 李静, 等. 嗜杀酵母的生物学功能及其应用[J]. 生物技术通报, 2013(04):33-38.
[35]李明春, 姜恒, 侯文强, 等. 酵母菌对重金属离子吸附的研究[J]. 菌物系统, 1998(04):80-86.