Abstract: To explore the scientific nitrogen application on winter wheat, field experiments were conducted to study the effect of different rates of controlled-release urea and conventional urea under the application of recommended total nitrogen (196 kg N/hm2) and nitrogen reduction 20% (156.8 kg N/hm2) on yield and nitrogen uptake and utilization of winter wheat. The results showed that the application of nitrogen fertilizer significantly increased winter wheat yield by 17.11%-27.39%, and the ecommended nitrogen application increased the yield by 2.14%- 8.77% compared with that of farmers’habits. The yield had no significant difference between the 20% of nitrogen reduction and total nitrogen. Under the condition of equal nitrogen, the nitrogen utilization efficiency of controlled release urea was higher than that of conventional urea, and that of nitrogen reduction was higher than that of total nitrogen treatment. The yield under nitrogen 156.8 kg/hm2 (75% controlled release urea and 25% conventional urea) increased by 6.60% compared with that of the farmers’ habits, and the nitrogen use efficiency increased by 13.64 and 9.22 percentage points compared with the empirical fertilization and recommended total nitrogen, and the agronomic efficiency of nitrogen fertilizer increased by 2.29 and 1.44 kg/kg. In conclusion, nitrogen 156.8 kg/hm2 (75% controlled-release urea and 25% conventional urea) used one time as basic fertilizer has good yield increasing effect on winter wheat with enhanced nitrogen fertilizer efficiency in the northern areas of Henan Province.