Abstract: To monitor avermectin, pyridaben, spirodiclofen, hexythiazox and etoxazole residues in citrus, a multi- residue analysis method for five acaricide residues in citrus was developed and validated by using QuEChERS processing with HPLC-MS/MS. The results showed that the purification by QuEChERS treatment method was ideal in the three citrus samples of Guanxi pomelo, ponkan and navel orange. The five kinds of acaricides showed a good linear relationship in the range of 0.5-500.0 μg/L (r 2>0.999), the LOD was 0.01- 0.30 μg/kg and the LOQ was 0.05-0.90 μg/kg. The additive recovery test results suggested that the additive concentrations of avermectin, pyridaben, hexythiazox and etoxazole of 2.0-200.0 μg/kg and spirodiclofen of 5.0-200.0 μg/kg were suitable to the requirement of pesticide residue analysis. The method is simple, fast, and has high sensitivity, good reproducibility and low detection concentration, so it can meet the requirements of maximum residue limit (MRL) in China and multi-residue detection.