Testis of Male Tilapia Under Methomyl Stress: Transcriptome Changes and Signal Pathway Analysis

doi:10.11924/j.issn.1000-6850.casb18040125

Abstract

Abstract: 369 significantly expressed genes were obtained by RNA sequencing(RNA-Seq). 160 genes were significantly down- regulated, and 209 genes were up-regulated. The expression of the differentially expressed genes in MAPK signaling pathway and ECM-receptor interaction was more significant. The results indicated that the adhesion of Testis tissue cells was influenced by Methomy stress, that induced the expression of related gene in ECM-receptor interaction to up-regulated for stabilizing the structure of testis tissue, such as Laminins, integrin alpha 6 and integrin beta 1. JNK, MAP3K and MKK4 in JNK pathway were significantly up-regulated, this enhanced transcription factor AP-1 to promote the expression of P53, Bax and other pro-apoptotic proteins. Because the expression of related growth factors in the ERK pathway of MAPK signaling pathway was significantly down-regulated by methomy stress, the proliferation of reproduction cells in the testis tissue may be affected.

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