Abstract: In order to solve the problems of low efficiency, high mutation and long period in rice genetic transformation, this paper first reported a piece of new equipment for vacuum infiltration transformation and its use method in rice. Rice variety ''Yunzijing 41'' was used as receptor in transformation. The whole rice plants at flowering stage were put inside the vacuum infiltration equipment. The young panicles in vivo were dipped in agrobacterium infection buffer. Then the agrobacterium-mediated transformation was carried out and enhanced by short time vacuum infiltration. In this experiment, a total of 30 rice plants were transformed and 19,860 T0 seeds were harvested. It was found that different concentrations of herbicides had no significant effect on the germination of T0 seeds, but had significant inhibitory effects on the growth and development of T1 plantlets. Therefore, the resistant transformants were selected by spraying herbicide at the seedling stage. The optimal screening concentration was 20 Mg/L. 135 resistant rice plants were obtained by herbicide resistance screening. 114 positive rice plants were obtained by PCR confirmation. It indicated that the foreign DNA has been successfully introduced into the recipient rice genome. This study first used the vacuum infiltration method and successfully introduced the exogenous genes into rice. That means we had developed a rapid and effective new way as well as a piece of equipment for gene transformation in rice and other cereal crops.