Abstract: The aim is to study the effect of different nitrogen applications on the yield and quality of buckwheat, and screen the optimum fertilizer application amount in buckwheat planting in the eastern farming areas of Qinghai. Field experiments were conducted by using sweet buckwheat‘Liuqiao1’and tartary buckwheat‘Heifeng1’; the growth period, yield and the components of yield were detected under 5 nitrogen levels; meanwhile, the seed quality was also analyzed. The results showed that, with the increase of the nitrogen, the yield and quality of the two buckwheat varieties first increased and then decreased; the best performance appeared when the nitrogen was 90 kg/hm2. For tartary buckwheat‘Heifeng1’, the plant height, the number of main stem brunches and the number of main stem nodes were significantly higher compared with CK (P<0.05), increasing by 23.21%, 15.63%, 9.80% respectively; while those of sweet buckwheat‘Liuqiao1’ increased by 8.48% , 15.29% and 14% respectively. Also, the components related to the yield increased greatly, the yield performance was the best when the nitrogen application was 90 kg/hm2, the yield was also the highest, and the differences of yield among varieties were relatively large. In quality, the 90 kg/hm2 treatment increased the protein content, starch content, crude fat content and flavone content of the two buckwheat varieties, which was significantly higher than that of CK. Therefore, under the experimental conditions, the nitrogen 90 kg/hm2 treatment has a good promoting effect on the yield and quality of buckwheat, and it is worth popularizing and applying.