Abstract: To study the regulation effect of spraying biological micro-fertilizer‘Saitufeng’on plant growth and yield improvement at pre- sowing stage, field experiments were conducted during 2016 to 2017. The experiment involved the wheat cultivar‘Zhengmai379’and three treatments: blank control (T1), water (T2), ‘Saitufeng’(T3). The agronomic characters such as plant height, the number of secondary root, leaf color, diseases and insect pests were recorded during the growth stage and the yield and yield components were examined at maturity stage. The results showed that‘Saitufeng’played an active role in increasing stem height, and protecting wheat plants from diseases and insect pests, especially promoting the development of root. Further analysis found that, compared with the treatment of water, the thousand grain weights and grain yields of‘Saitufeng’treatment in two experimental sites increased significantly by 13.09% , 12.44% and 4.67% , 4.64% , respectively. The spike number and grain number increased but not significantly. Thus, spraying‘Saitufeng’on soil at pre-sowing could improve the yield by increasing the grain weight, and the effect is obvious.