Abstract: To study the effect of different transplanting time and topping modes on the quality of‘CB-1’ tobacco leaf in different altitude areas of Fujian Province, field randomized block tests were conducted. The results showed that in 250 meters altitude area, with the delay of transplanting time and multi-topping period, the growth duration of tobacco was shortened, the plant height was increased, the incidence rate went up, the yield and quality of flue-cured tobacco was deteriorated, and the coordination of chemical components and the sensory quality was decreased. In 650 meters altitude area, with the delay of transplanting time and multitopping period, the growth duration of tobacco was shortened, no significant changes in agronomic traits were observed, the incidence rate had no obvious change, the yield and quality of flue-cured tobacco, and the coordination of chemical components and the sensory quality were first increased and then decreased. To improve the yield and quality of flue- cured tobacco, in 250 meters altitude area of Fujian Province, the planting of‘CB-1’should be carried out by earlier transplanting (January 10) and initial flowering topping (10% central flower development). In the 650 meters altitude area of Fujian Province,‘CB-1’should be cultivated by mid-term transplanting (February 4) and full flowering topping (40% central flower development).