Abstract: To illuminate the florfenicol resistance of bacteria from swine and genetic characteristics of floR gene in Ningde area of Fujian province, some experiments were maked. In this study, 50 piglet feces samples were collected from 6 pig farms in Ningde area. 17 strains of florfencol resistant Escherichia coli, 2 Salmonella isolates, 5 strains of Klebsiella pneumoniae and 8 Acinetobacter baumannii strains were isolated from these feces. And the homology of floR gene from the bacteria isolated was analyzed. The results showed the resistance rates of Escherichia coli, Salmonella, Klebsiella pneumoniae, and Acinetobacter Baumannii to florfenicol in Ningde region were 34%, 4%, 10% and 16%, respectively. And all the isolated bacteria showed multiple resistance. Homology analysis revealed that floR was highly homologous among different bacteria. These results indicated that the florfenicol resistant gene floR exists widely among different bacteria and can be transmitted horizontally between different bacteria. The study laid a theoretical foundation for the wide spread of florfenicol resistant gene floR and provided a basis for clinical rational drug use.