Effects of Light Quality of Light Emitting Diode (LED) on Callus Induction and Regeneration of Indigoblue Woad

doi:10.11924/j.issn.1000-6850.casb18070064

Abstract

Abstract: To study the effects of light quality of light emitting diode (LED) on callus induction and regeneration of indigoblue woad, the seeds of indigoblue woad (Isatis indigotica Fort)‘Dinglan1’were used as the material. Six light treatments were set as follows: fluorescent lamp (CK), 100R, 100B, 80R/20B, 70R/30B and 50R/50B. The contents of chlorophyll and soluble sugar were detected. The results showed that the complex light of the red and blue LED had better effect compared with only red or blue LED. Under 70R/30B treatment, the callus of indigoblue woad grew best. It was green and had close texture. The differentiation rate was 68.75%. The rooting rate of regenerated plants was 92.86%. The rooting status was characterized by early rooting, thick root and long root under 70R/30B, which were significantly higher than those of the control. Under 70R/30B treatment, the content of chlorophyll a, total chlorophyll and soluble sugar reached the maximum, 0.497 mg/g, 0.654 mg/g and 0.244 mg/g respectively. In a summary, it suggests that the complex light of the red and blue LED is suitable for the induction and differentiation of callus, morphogenesis of regeneration plant, chlorophyll synthesis and soluble sugar accumulation. 70R/30B is the best light.

References

[1] 王晓宇, 赵晓明.S不同倍性菘蓝根叶干物质积累特性研究初报[J].S中国农学通报,S2008,S24(3): 258-261.
[2] 孙强, 王丽艳, 荆瑞勇,等. 不同倍性菘蓝对干旱胁迫的耐受能力及保护性酶的响应机制[J]. 水土保持研究, 2013, 20(6): 48-53.
[3] 客绍英. 菘蓝组培快繁体系的建立及四倍体株系选育和品质鉴定[D]. 北京:北京林业大学, 2010.
[4] 刘丽萍. 菘蓝离体再生体系的建立及多倍体诱导[D]. 大庆:黑龙江八一农垦大学, 2008.
[5] 陈桂平, 客绍英. 二倍体及四倍体菘蓝氨基酸含量的测定分析[J]. 中药材, 2011, 34(4): 506-508
[6] 客绍英. 菘蓝组培快繁体系的建立及四倍体株系选育和品质鉴定[D]. 北京:北京林业大学, 2010.
[7] 吴启藩. 不同LED光源对长茎葡萄蕨藻生长、生理生化特性及品质的影响[D].湛江：广东海洋大学, 2017.
[8] 齐连东, 刘世琦. 光质对植物光形态建成、品质及延缓衰老的影响[C]// 中国园艺学会青年学术讨论会. 2006.
[9] 孟霖. 光质对烤烟叶片代谢物质积累及烟苗发育的影响[D]. 北京：中国农业科学院, 2015.
[10] 汪凤林, 曹光球, 叶义全,等. 不同光质下杉木幼苗叶片光合作用的光响应[J]. 森林与环境学报, 2017, 37(3): 366-371.
[11] 伊万伟, 武兴宝, 李方华,等. 不同光质对仙客来愈伤组织诱导和分化及生根的影响[J]. 上海蔬菜, 2017(3): 65-67.
[12] 闫新房, 丁林波, 丁义,等. LED光源在植物组织培养中的应用[J]. 中国农学通报, 2009, 25(12): 42-45.
[13] Bula R J. Light-emitting diodes as radiation sources for plants[J]. Hortscience, 1991, 26.
[14] Hoenecke M E, Bula R J, Tibbitts T W. Importance of ''blue'' photon levels for lettuce seedlings grown under red-light-emitting diodes.[J]. Hortscience A Publication of the American Society for Horticultural Science, 1992, 27(5): 427.
[15] 曹刚, 张国斌, 郁继华,等. 不同光质LED光源对黄瓜苗期生长及叶绿素荧光参数的影响[J].中国农业科学, 2013, 46(6): 1297-1304.
[16] 李雯琳, 郁继华, 张国斌,等. LED光源不同光质对叶用莴苣幼苗叶片气体参数和叶绿素荧光参数的影响[J]. 甘肃农业大学学报, 2010, 45(1): 47-51.
[17] 陈颖, 王政, 纪思羽,等. LED光源不同光质比例对红掌试管苗生长的影响[J]. 江西农业大学学报, 2013, 35(2): 375-380.
[18] 刘庆, 连海峰, 刘世琦,等. 不同光质LED光源对草莓光合特性、产量及品质的影响[J]. 应用生态学报, 2015, 26(6): 1743-1750.
[19] Arnon D I. Copper enzymes in isolated chloroplsts. polyphenoloxidase in beta vulgaris.[J]. Plant Physiology, 1949, 24(1): 1-15.
[20] 刘海英, 王华华, 崔长海,等. 可溶性糖含量测定(蒽酮法)实验的改进[J]. 实验室科学, 2013, 16(2): 19-20.
[21] De C.Action of light and temperature on growth of plant tissue culture in vitro[J].Am J Bot,1955,42: 869-873.
[22] 马琳, 刘世琦, 张自坤,等. 光质对大蒜愈伤组织诱导、增殖及器官分化的影响[J]. 西北农业学报, 2011, 20(6): 118-122.
[23] 周吉源, 赵洁, 程井辰. 油菜薄层细胞培养中不同光质对愈伤组织诱导和增殖的效应[J]. 植物学报, 1992(1): 77-81.
[23] Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of Chrysanthemumplantlets in vitro.ScientiaHorticulturae 101: 143–151
[24] 王丽伟, 李岩, 辛国凤,等. 不同比例红蓝光对番茄幼苗生长和光合作用的影响[J]. 应用生态学报, 2017, 28(5): 1595-1602.
[26] 刘文科, 杨其长, 邱志平,等. LED光质对豌豆苗生长、光合色素和营养品质的影响[J]. 中国农业气象, 2012, 33(4): 500-504.
[27] 尚文倩, 王政, 侯甲男,等. 不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响[J]. 西北农林科技大学学报(自然科学版), 2013, 41(5): 155-159.
[28] 王丽娟, 白义奎, 王铁良. 不同光质处理对草莓生理特性的影响[J]. 河南农业大学学报, 2017(1): 25-28.
[29] 石镇源, 唐敏, 杨红飞, 等. LED不同光质对虎雪兰组培苗生理生化特性影响的研究[J]. 云南农业大学学报:自然科学版, 2012, 27(6): 863-869.
[30] Kasperbauer M J. Strawberry yield over red versus black plastic mulch.[J]. 2000, 40(1):171-174.