The Structure, Function Prediction of 14-3-3 Protein and Its Expression at Maturity Stage of Cassava

doi:10.11924/j.issn.1000-6850.casb18070142

Abstract

Abstract: This study aims to lucubrate our research on structures and functions of 14-3-3 protein family, as well as to provid a new insight into the molecular regulation of starch accumulation in cassava tuberous roots.The 14-3-3 proteins are signal transduction factors in plants, which play an important role in regulating starch synthesis in cassava tuberous roots. In the present study, the sequences composition, physicochemical properties, phylogenetic evolution, leader peptides, subcellular localization, the secondary structures, functional domains, tertiary structures and hydrophobicity/hydrophilicity of cassava 14-3-3 protein family were predicted and analyzed using bioinformatics tools. Differential expressions of 14-3-3 genes in different cassava cultivars were analyzed. The result showed that (1) cassava 14-3-3 protein family consisting of 16 isoforms had multiple conservative structure domains and these hydrophilic proteins, which are similar in the secondary structure, were classified into two different categories based on their evolutionary relationship, (2) there were significant differences in the expressions among 16 members from 14-3-3 gene family in the tuberous roots of the mature stage of mosaic cassava, ZM-Seaside and SC9 (P<0.05), and the expression of 14-3-3 gene family in cassava variety ZM-Seaside with high yield decreased significantly (p<0.05) compared to mosaic cassava and SC9 (P<0.05), inferring the 14-3-3 proteins may play a negative-regulation role in starch accumulation of the mature stage of cassava tuberous roots.

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