Optimization of Extraction Process of Polysaccharide of Phellinus igniarius Mycelium and Analysis of Its Antioxidant Activity in vitro

doi:10.11924/j.issn.1000-6850.casb18080057

Abstract

Abstract: To study extraction process of polysaccharide from Phellinus igniarius mycelium and its antioxidant activity in vitro, according to hot water extraction and based on the single factor experiments, the extraction condition of polysaccharide from Phellinus igniarius was optimized using orthogonal test and its antioxidant activity in vitro was primarily evaluated by ABTS+. and DPPH. free radical scavenging ability. The results showed that the optimal extraction conditions were determined as follows: extraction time 2.5 hours, extraction temperature 60 ℃, number of extraction 3 times and liquid-to-solid ratio of 14 times. Under these conditions, the average extraction yield was 4.97%. The result of antioxidant activity in vitro experiments showed that the highest scavenging ability of polysaccharides against ABTS+. and DPPH. was 73.54% and 88.83% respectively. The optimization extraction process of polysaccharide from Phellinus igniarius Mycelium was reasonable and feasible, and it had stronger antioxidant activity and had a potential exploited value in functional foods and medicines.

CLC Number:

TS201.1

References

[1]刘波.中国药用真菌[M].太原:山西人民出版社,1974.
[2]魏静,陈力,邓亚君,等.液体发酵的桑黄多糖对小鼠免疫功能的影响 [J].食品工业科技,2016,37(8):344-346.
[3]Kim G Y, Lee J Y, Lee J O, et a1. Paritial characterization and immunostimulatory effect of a novel polysaccharide-protein comLex extracted from Phellinus linteus, Biosci Biotechnol Biochem [J]. 2006, 70(5):1218-1226.
[4]Lee W Y, Hsu K F, Chiang T A, et al. Phellinus linteus extract induces autophagy and synergizes with 5-fluorouracil to inhibit breast cancer cell growth [J]. Nutrition Cancer, 2015, 67(2):275.
[5]孙佳琦,张甘霖,于明薇,等. 桑黄成分及其抗肿瘤作用机制研究进展 [J].中国医药导报,2016,13(33):39-42.
[6]Li Y G, Ji D F, Zhong S, et al. Anti-tumor effects of proteoglycan from Phellinus linteus by immunomodulating and inhibiting Reg IV/EGFR/Akt signaling pathway in colorectal carcinoma [J]. International Journal of Biological Macromolecules, 2011, 48(3):511-517.
[7]丁云云,刘锋,施超,等.桑黄化学成分及体外抗肿瘤活性研究 [J].中国中药杂志,2016,41(16):3042-3048.
[8]李世超. 桑黄菌丝体多糖的分离纯化、活性研究和结构分析 [D]. 镇江:江苏大学,2015.
[9]李婷婷,杨焱,刘艳芳,等.超微粉碎对桑黄子实体粗多糖理化性质和刺激巨噬细胞活性的影响 [J].天然产物研究与开发,2015(2):333-337.
[10]吕方冰,张娜,俞淑文.桑黄多糖抗肿瘤机制研究进展 [J].上海中医药杂志,2015(9):87-89.
[11]Senovilla L, Aranda F, Galluzzi L, et al. Impact of myeloid cells on the efficacy of anticancer chemotherapy [J]. Current Opinion in Immunology, 2014, 30:24-31.
[12]孟庆龙,潘景芝,陈丽,等.桑黄胞内及胞外多糖对荷瘤小鼠减毒增效作用的研究 [J].中国中药杂志, 2012,37(6):847-852.
[13]Sideras K, Braat H, Kwekkeboom J, et al. Role of the immune system in pancreatic cancer progression and immune modulating treatment strategies [J]. Cancer Treatment Reviews, 2014, 40(4):513-522.
[14]张林芳,邹莉.桑黄多糖的研究进展 [J].中国食用菌,2012,31(4):1-4.
[15]Pei J J, Wang Z B, Ma H L, et al. Structural features and antitumor activity of a novel polysaccharide from alkaline extract of Phellinus linteus, mycelia [J]. Carbohydrate Polymers, 2015, 115:472-481.
[16]Yan J K, Pei J J, Ma H L, et al. Advances in antitumor polysaccharides from Phellinus Sensu Lato: production, isolation, structure, antitumor activity, and mechanisms [J]. Critical Reviews in Food Science Nutrition, 2017, 57(6):1256-1269.
[17]钟石,李有贵,林天宝,等.桑黄多糖P1对肝癌HepG2细胞周期和钙调蛋白信号通路的影响 [J].中国药理学与毒理学杂志,2015,29(3):417-423.
[18]李瑞雪,王钰婷,夏家凤,等. 桑叶总黄酮提取工艺优化及其抗氧化活性分析 [J].北方蚕业,2017,38(4):4-11.
[19]邹湘月,李飞鸣,邵元元,等.桑枝水提物对桑黄液体发酵的影响及桑黄液体发酵条件的优化研究 [J].中国蚕业,2016,37(4):17-20.
[20]吴亚召,雷萍,张文隽,等.桑黄黄酮液体发酵培养基的优化 [J].中国食用菌, 2016,35(5): 21-23.
[21]李六文,赵刚.药用真菌多糖抗肿瘤免疫生物活性研究进展 [J].中华肿瘤防治杂志,2015,22(14): 1156-1160.
[22]王立娜,陈喜君,王辉,等.复合药用真菌多糖抗肿瘤活性的研究进展 [J].生物技术通讯,2016,27(6): 888-891.
[23]雷萍,张文隽,吴亚召,等.桑树桑黄子实体和发酵菌粉有效成分分析 [J].中国食用菌,2010,29(4):40-42.
[24]史帧婷,包海鹰.桑黄类真菌有效成分及功效研究进展 [J].中国实验方剂学杂志,2016(22):197-202.