Abstract: The distribution characteristics of Cu, Pb, Cr, Cd, As and Hg in tobacco leaves from different regions of Qujing tobacco-growing area and the differences between different flue-cured tobacco varieties and different altitudes were analyzed. The results showed that the content of Pb in flue-cured tobacco in Qujing tobacco area was at a high level, and Cu, Cr, Cd, As and Hg were at a low level. Pb is probably the main heavy metal element in flue-cured tobacco in Qujing. The content of heavy metals in flue-cured tobacco was significantly different among different counties in Qujing. The accumulation of heavy metals in flue-cured tobacco was less in Shizong and Luliang, and more in Zhanyi, Xuanwei and Qilin. The content of Pb in Zhanyi was significantly higher than that in other counties, and the proportion of heavy metals in Zhanyi was as high as 100% of the average sample in Qujing. The contents of heavy metals were significantly different among different varieties. The contents of Cu in Yunyan 97 and Cd in Yunyan 105 were the lowest, the contents of Pb in Hongda and Cr in Yunyan 100 were the highest, while the contents of As and Hg were not significantly different among different varieties. The relatively high content of heavy metals in tobacco leaves is Hongda, while Yunyan 97 and K326 are relatively low. The differences of Cu, Cd, Cr and As in flue-cured tobacco did not reach significant levels in different altitude ranges. The contents of Pb in flue-cured tobacco at 2000-2200m and Hg at 1400-1600m were significantly higher than those at other altitudes.