Cercospora Leaf Spot (CLS) of sugar beet significantly influences the yield and quality of sugar beet, and spraying fungicide is a key method to prevent sugar beet from CLS. To prevent CLS, from 2015-2016, five treatments (spraying times: 4, 3, 2, 1, and water as control), four repeats, randomized block design were used. The control effects, root yield, sugar rate and sugar content were investigated, and the optimal prevention time was determined. The results of two years’study showed that the differences among the treatments were significant. However, there was no significant difference of root yield among the treatments. Furthermore, sugar rate of every treatment was significantly different in 2015, but it was not significantly different in 2016, and sugar content ditto. The results exhibit ed that the control effect, root yield, sugar rate and sugar content increased with the increasing of spraying times. All the indexes of 4 spraying times were the highest, and the control effect was the best. Control effect, root yield, sugar rate and sugar content of 4 spraying times in two years were 70.54%, 7.23 kg/m2, 14.91% and 1.15 kg/m2.