Abstract: Compared with the Middle East of China, the atmospheric environment in Guiyang is cleaner, and the change of O3 needs further study. In this paper, the hourly concentration data , meteorological and geographic data of O3 in 10 air quality monitoring points set up by Guiyang Environmental Protection Bureau during the past five years (January 2013-December 2017) were analyzed, and the temporal and spatial distribution characteristics of O3 concentration in Guiyang were discussed. In this paper, the spatial and temporal variations of ozone in Guiyang city were analyzed mainly from the special topography of the basin and wind effects, aiming at the high concentration of ozone relative to other pollutants and the high concentration of ozone at night in suburbs. The results show that: (1) The annual average concentration of O3 in Guiyang city increases year by year, which is related to the increase of emission sources. The seasonal variation of O3 is mainly affected by weather and climate conditions, and the variation of O3 is significantly related to global solar radiation、air temperature and so on. (2) The concentration of O3 in suburb is obviously higher than that in urban area, and the time of occurrence is mostly at night. This phenomenon is related to the difference of emission sources between urban and suburban areas, the nature of underlying surface and the characteristics of topography and microclimate. (3) Under certain emission sources, the main factors affecting the variation of O3 concentration in urban and suburban areas of Guiyang are special basin topography and wind. The change hour of O3 is positively correlated with wind speed in winter half year and summer half year. The variation of O3 in the suburbs is also positively correlated with wind speed in the winter half year, but in the summer half year, the hour concentration of O3 in the suburbs is positively correlated with wind speed, which is caused by the valley topography and wind direction in the suburbs.