Expression Profiling of Cold-responsive MicroRNA in Banana

doi:10.11924/j.issn.1000-6850.casb18100007

Abstract

Abstract: To explore the potential roles of miRNA in banana under cold stress, weSanalyzed their dynamic expression of cold responsive miRNAs in banana. The expression profiling of cold-responsive miRNAs in banana leaves was detected by stem-loop qRT-PCR after 0, 2, 4, 8 , 12 and 24 h of cold stress at 5&#176;C. The results showed that fifteen potential cold-responsive miRNA were expressed and exhibited different expression patterns at different treatment stages. The 15 miRNA were divided into four groups according to their expression pattern. Generally, the expressions of Group I (miR159e, miR159f, miR160-3p, miR397a, miR399j, miR408, miR5179, miR530, miR535 and miR5538) and II (miR164e and miR444a) were upregulated at 5&#176;C cold stress. During the cold stress, the average expression level of these miRNA was higher than the control (0 h). miR156l, miR162a and miR166a were in Group III, and their expression was reduced during the whole period of the cold treatment. Group IV had only one miRNA (miR156a). Its expression was significantly increased only at 12 h and reduced at the rest of the time points under cold stress. The analysis on the target genes of these miRNA revealed that these miRNA participated in the signal transduction, growth and development and stress response under abiotic stress. These findings provide valuable information for further functional characterization of miRNA associated with cold stress in banana.

CLC Number:

S668.1

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