Abstract: The objective of this study was developed in order to excavate excellent germplasm resources for cultivation of high resistance to Cercospora sojina. The disease index of soybean to Cercospora sojina was identified using 1164 portions (915 from China, 145 from outside and 104 from wild germplasm) by artificial inoculation. The average disease index of 1164 portions to Cercospora sojina was 52.2%, Genetic coefficient of variation was 23.9%; 64.9% of all varieties has the resistance to disease. ‘Chengduo 6’ was the highest-quality disease-resistant varietie which the disease index was 10%; The resistance of foreign soybean varieties to Cercospora sojina was higher than that of wild and domestic germplasm resources. The average disease index of Heilongjiang province varieties to Cercospora sojina was 52.0%, which resistance to disease was higher than other 13 provinces soybean population in china. There were 7 main soybean series varieties in Heilongjiang province. 93.8% varieties of ‘Kennong’ series showed the resistance to disease. 88.2% varieties of ‘Kenjiandou’ series showed the resistance to disease, 85.7% varieties of ‘Suinong’ series showed the resistance to disease. Ten varieties were screened out from Heilongjiang province soybean population. They were ‘Kennong 19’, ‘Kennong 17’, ‘Heinong 43’, ‘Heinong 47’, ‘Kenjiandou 4’, ‘Kenjiandou 33’, ‘Suinong 11’, ‘Suinong 12’, ‘Suinong 15’ and ‘Dongnong 43’. The resistance of soybean germplasm resources to Cercospora sojina retains abundant genetic variation. 10 varieties could be utilized inbreeding programs which aim to develop varieties resistance to Cercospora sojina.