Abstract: The paper aims to study the soil microorganism associated with konjac soft rot disease. A dominant strain named ZX001 was isolated from the recesses of rotten corm of Amorphophallus Konjac infected with soft rot disease. We compared the 16S rDNA sequence of the strain with gene sequences in the NCBI database. The results showed that: the gene sequence was closely related to the Bacillus megaterium strain (99%); combined with the results of morphological, physiological, and biochemical characteristics, the ZX001 strain was preliminary identified as a Bacillus megaterium; subsequent experiments showed that ZX001 could not grown on the surface of konjac corm pericarp, but could grown on the sarcocarp of konjac corm, and had the ability to degrade and utilize konjac glucomannan. The results indicate that this strain is not a pathogenic bacterium of the konjac soft rot disease, but it might enter into konjac corm through the wound on the surface of pericarp, and may produce many enzymes to degrade konjac corm sarcocarp, so as to aggravate the decay of konjac corm, and increase the chance of soft rot disease.