Abstract: Androgenesis is a process, which microspores or immature pollen cells regenerate plants by the sporophytic development pathway, and that is one of the effective ways to create breeding materials. To establish the stable microspore culture method, with higher regeneration rates, this paper reviewed the domestic and foreign research results, summarized the main influence factors of androgenesis stages (obtainment of embryogenesis, induction of embryos and regeneration of haploid plants), and concluded the culture techniques of isolating microspores. The analysis showed that genotype was restrictive?factors, and androgenesis could deviate from the gametophyte pathway, when the influence factors appear at the same time. In the end, we discussed the future research direction of microspore culture, and expected to offer reference for the deepening research of doubled haploid technology in tomato.