Effects of Drought Stress on Active Oxygen Metabolism, Osmotic Regulators, SPAD and Chlorophyll Fluorescence Characteristics of Sweet Potato

doi:10.11924/j.issn.1000-6850.casb19030085

Abstract

Abstract: To explore the response mechanism of sweet potato to drought stress and its growth water requirement, the effects of different drought intensities (0% , 6% , 12% , 18% and 24% of PEG6000 concentration respectively) on active oxygen metabolism, osmotic regulators, SPAD and chlorophyll fluorescence characteristics of sweet potato were studied by soilless cultivation with‘Shangshu 19’as experimental material. The results showed that the activities of protective enzymes SOD, POD, CAT, APX, soluble protein and soluble sugar first increased and then decreased with the increase of drought stress intensity, and the activities of protective enzymes SOD, POD, CAT and APX increased when the concentration of PEG-6000 was not higher than 7%. The content of soluble protein and soluble sugar increased by 58.09% and 161.14% respectively when the concentration of PEG- 6000 was 7% . The content of proline and malondialdehyde increased with the increase of drought stress intensity. When the concentration of PEG-6000 was not higher than 3% , it could stimulate the chlorophyll production of sweet potato leaves. SPAD was negatively correlated with the intensity of drought stress. The fitting equation of SPAD and drought stress was y=54.354e-3.323x. Initial fluorescence (Fo) showed the change rule of first decreasing and then increasing, and the lowest value was at 3% PEG-6000 concentration; the maximum fluorescence (Fm), maximum photochemical efficiency (Fv/Fm) and photochemical extinction coefficient (qP) showed a single peak change rule of first increasing and then decreasing with the increase of drought stress intensity, and the maximum value was at 3% PEG-6000 concentration, which had a negative correlation with initial fluorescence (Fo). It is concluded that water-saving irrigation could be achieved under the condition of maintaining healthy growth of sweet potato seedlings when the soil moisture content is kept at 3% PEG-6000 concentration, but the soil drought intensity could not exceed 7% of PEG- 6000 concentration, otherwise, the morphological and physiological characteristics of sweet potato seedlings would be adversely affected.

References

[1]李博.几种玉簪的水分胁迫耐受性研究[D].哈尔滨:东北林业大学,2008.
[2]山仑.旱地农业技术发展趋向[J].中国农业科学,2002, 35(7):848-855.
[3]何梅,施大伟,胡玉安,等.干旱胁迫下银杏雌雄植株的生长及内源激素含量的差异[J].江西农业大学学报,2017,39(6):1154-1162.
[4]李滨胜,周玉迁,潘杰,等.干旱胁迫下细叶景天生理生化指标的变化[J].北方园艺,2010(16):105-107.
[5]马洪波,李传哲,宁运旺,等.钙镁缺乏对不同甘薯品种的生长及矿质元素吸收的影响[J].中国土壤与肥料,2015(4):101-107.
[6]赵世杰,史国安,董新纯.植物生理学实验指导{Ml.北京:中国农业科学技术出版社,1995.
[7]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[8]Rohacek K. Chlorophyll fiuorescenceparameters:The definitions photosynthetic meaning and mutual relationships[J].Photosynthetica,2002,40(1):13-29.
[9]严子柱,李得禄,满多清,等. PEG-6000模拟干旱胁迫对铃铛刺和披针叶野决明种子萌发的影响[J].干旱区研究,2017,34(1):88-94.
[10]李合生.现代植物生理学[M].北京:高等教育出版社,2002:415-419.
[11]Giannopolitis C N,Ries S K.Superoxide dismutase II.Purification and quantitative relationship with water soluble protein in seedlings[J].Plant Physiology,1977,59 (6):315-318.
[12]Ahmed B R B B, Sensoy S, Boukhris M, et al.. Changes in gas exchange, proline accumulation and antioxidative enzyme activities in three olive cultivars under contrasting water availability regimes[J].Environmental and Experimental Botany,2009,67(2): 345-352.
[13]Al-Ghamdi A A. Evaluation of oxidative stress tolerance in two wheat (Iriticum aestivum) cultivars in response to drought[J].lnternational Journal of Agriculture Biology. 2009,11(1):1560-8530.
[14]常红军,刘兰霞.植物抗旱分子机理研究进展[J].安徽农业科学,2006, 34(18). 4509-4510, 4514.
[15]王忠.植物生理学[J].北京:中国农业出版社,2000.
[16]罗爱华,李文甲.干旱胁迫对番茄扦插苗叶片丙二醛、脯氨酸含量及保护酶活性的影响[J].园艺与种苗,2018(02):17-20,49.
[17]魏良民.几种旱生植物碳水化合物和蛋白质变化的研究[J].干旱研究,1991, 8 (4): 38-41.
[18]韩忠明,胥苗苗,王云贺,等.干旱胁迫对防风叶片保护酶活性、渗透调节物质含量及药材品质的影响[J].华南农业大学学报,2016,37 (6) :91-97.
[19]Azarabadi S,Abdollahi H,Torabi M A,et al. ROS Generation,oxidative burst and dynamic expression profiles of ROS scavenging enzymes of superoxide dismutase(SOD),catalase(CAT)and ascorbate peroxidase(APX) in response to Erwinia amylovora in pear(Pyruscommuuis L)[J]. European Journal of Plant Pathology,2017,147(2):279-294.
[20]王继玥,石登红,白禹,等.PEG-6000模拟干旱胁迫对黄秋葵种子萌发和幼苗生理特性的影响[J].热带亚热带植物学报,2018, 26(6): 611-616.
[21]潘瑞炽,王小菁.李娘辉.植物生理学(第六版)[M].高等教育出版社,2008,61-66.
[22]谢美华,李雪玲,杨海燕,等.干旱胁迫对不同玉米品种幼苗叶片SPDA值影响的研究[J].楚雄师范学报,2014,29(6)：32-35.
[23]刘燕燕,张聘,曹昀,等.对紫穗槐种了萌发及幼苗生长的影响[J].江苏农业科学,2014,42(9)：145-147.
[24]高蕾.干旱胁迫对大豆幼苗叶片生理生化特性的影响[J].东北农业大学学报,2009,40(08):1-4.
[25]李清芳,马成仓,尚启亮.干旱胁迫下硅对玉米光合作用和保护酶的影响[J].应用生态学报,2007,18(3):531-536.
[26]胡文海,喻景权.低温弱光对番茄叶片光合作用和叶绿素荧光参数的影响[J].园艺学报,2001, 28(1):41-46.
[27]段娜,贾玉奎,郝玉光,等.干旱胁迫对欧李叶绿素荧光特性的影响[J].西北林学院学报2018,33(6): 10-14.
[28]G N JOHNSON, A J YOUNG, J D SCHOLES, et al.. The dissipation of excess excitation energy in British plant species[J].Plant,Cell and Environment, 1993, 16,673-679.
[29]葛玮.不同强度干旱胁迫对巨龙竹光合作用及叶绿素荧光特性影响[J].宁夏林业,2018,6：40-45.
[30]刘筱,王锐洁,杨淑君,等.干旱胁迫对何首乌生长和叶绿素荧光参数的影响[J].河南农业科学,201948 (1) :50-56.
[31]童小芹,土淑智,夏咏,等.应用叶绿素荧光技术快速预警乌鲁木齐典型农作物干旱胁迫[Jl.干旱区研究,2013, 30(5):860- 866.