Abstract: This study aims to obtain the new fungal resources with activities from forest litters of the Greater Khingan Mountains. A particle method was used to isolate cultivable fungi from forest litters. The fungal sequences of internal transcribed spacer were used for the molecular identification of isolated fungi. Two methods were used for the fermentation of novel fungi and the preparation of secondary metabolic extracts. A double-layer-plate drug diffusion assay and a DPPH radical scavenging assay by thin layer chromatography were used to test activities of crude extracts, respectively. The result of molecular identification showed that 40 fungi were isolated and cultured, and they belonged to 35 genera and 38 taxa, of which 6 fungi were suspected as new fungal species. 2 crude extracts from 2 fungal strains showed the antibacterial activities. In particular, the strain LB0026 of Parapyenchaeta genus had stronger activity against a gram-positive bacterium and no significant activity against a tested gram-negative bacterium. In addition, other 2 crude extracts from 2 fungal strains showed the DPPH free radical scavenging activity. There were 2 and 3 antioxidant compounds in these 2 crude extracts, respectively. In the forest litters of the Greater Khingan Mountains, there are rich kinds of cultivable fungi, of which 4 strains of new fungi have obvious antibacterial or antioxidant activities and they are worthy of further study.