Abstract: In this study, tissue culture technology was used to establish a rapid propagation system of Aquilaria sinensis with the seeds. Firstly, seeds were sterilized and germinated on MS medium. Then, the sterile seedlings were cut into segments with one potential lateral bud to be induced to grow into cluster buds. Finally, the single growing-up plantlets were cut off for rooting. The best medium for differentiation was MS+ 1 mg/L 6-BA+0.2 mg/L NAA+ 0.2 mg/L IBA+ 30 g/L sucrose+ 5 g/L plant gel, and the best medium for rooting of tissue cultured plantlets was MS+ 30 g/L sucrose+ 5 g/L plant gel. The establishment of rapid propagation system for A. sinensis will be applied to the rapid massive propagation of the special germplasms of A. sinensis. In addition, due to the hard preservation of seeds of A. sinensis, the rapid propagation technology can also provide a large number of fresh and tender tissue materials with relatively identical genetic background for the study of agarwood formation mechanism.