Nitrogen Metabolic Characteristics of Two Cultivable Bacteria

doi:10.11924/j.issn.1000-6850.casb20191100789

Abstract

Abstract:

To study the N metabolic characteristics of culturable bacteria in soil, and discuss the possible mechanism of microorganisms in soil N transformation, 2 strains, named SY5-4 and SY11-10 isolated from apple orchard soil were selected to analyze the growth characteristics, and their capabilities of nitrogen transformation, by using the traditional culture method combined with molecular technology. The results showed that, in heterotrophic condition, the generation time of SY5-4 and SY11-10 was 243.5 min and 202.7 min, respectively. The concentration of NH₄⁺-N in the medium maintained at a high level, while the concentration of NH₄⁺-N, NO₂^--N and NO₃^--N increased first and decreased afterwards, in the process of bacterial growth. The nitrification (amoA and hao), and denitrification (nosZ, norB, nirK and nap) gene detection showed that, strain SY11-10 had a variety of N transformation potential. In conclusion, the nitrogen in medium changed in the process of bacterial growth, and different nitrogen transformation genes were detected in the tested strains, which indicated that the isolated strains participated in various N metabolism pathways.

Key words: culturable bacteria, heterotrophic medium, nitrogen metabolism, nitrification gene, denitrification gene

CLC Number:

S154.3

Liu Zhixin, Yao Na, Liu Lingzhi. Nitrogen Metabolic Characteristics of Two Cultivable Bacteria[J]. Chinese Agricultural Science Bulletin, 2020, 36(30): 106-111.

Figures/Tables 4

References 30

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引物名称与序列(5’-3’)	参考文献
amoA-1F: 5’-GGGGTTTCTACTGGTGGT-3’ amoA-2R: 5’-CCCCTCKGSAAAGCCTTCTTC-3’	[23]
nosZ-1870F: 5’-GCRACGGCAASAAGGTSMSSGT-3’ nosZ-2090R: 5’-CAKRTGCAKSGCRTGGCAGAA-3’	[24]
norB-1F: 5’- CGNGARTTYCTSGARCARCC-3’ norB-8R: 5’-CRTADGCVCCRWAGAAVGC-3’	[25]
nirK-1F: 5’-GGMATGGTKCCSTGGCA-3’ nirK-3R: ’-GAACTTGCCGGTVGYCCAGAC-3’	[26]
nap-1F: 5’-TCTGGACCATGGGCTTCAACCA-3’ nap-1R: 5’-ACGACGACCGGCCAGCGCA-3’	[27]
hao-1F: 5’-TGCGTGGARTGYCAC-3’ hao-3R: 5’-AGRTARGAKYSGGCAAA-3’	[28]

引物名称与序列(5’-3’)	参考文献
amoA-1F: 5’-GGGGTTTCTACTGGTGGT-3’ amoA-2R: 5’-CCCCTCKGSAAAGCCTTCTTC-3’	[23]
nosZ-1870F: 5’-GCRACGGCAASAAGGTSMSSGT-3’ nosZ-2090R: 5’-CAKRTGCAKSGCRTGGCAGAA-3’	[24]
norB-1F: 5’- CGNGARTTYCTSGARCARCC-3’ norB-8R: 5’-CRTADGCVCCRWAGAAVGC-3’	[25]
nirK-1F: 5’-GGMATGGTKCCSTGGCA-3’ nirK-3R: ’-GAACTTGCCGGTVGYCCAGAC-3’	[26]
nap-1F: 5’-TCTGGACCATGGGCTTCAACCA-3’ nap-1R: 5’-ACGACGACCGGCCAGCGCA-3’	[27]
hao-1F: 5’-TGCGTGGARTGYCAC-3’ hao-3R: 5’-AGRTARGAKYSGGCAAA-3’	[28]