Soybean Variety Resource Resistant to Cercospora sojina Race 7: Mining of Elite Alleles

doi:10.11924/j.issn.1000-6850.casb2020-0021

Abstract

Abstract:

The objective is to explore the elite allele of soybean variety resource resistant to Cercospora sojina race 7, and provide genetic information and carrier materials for breeding soybean varieties resistant to C. sojina. In this study, 202 commercial soybean varieties were used as the experimental materials, and the disease resistance to race 7 of C. sojina was identified. The whole genome scanning was used by SSR markers. Association analysis for soybean varieties’ resistance to C. sojina was performed by GLM and MLM model. The results showed that a total of 12 loci were detected by GLM and MLM model, which were significantly associated with the resistance to C. sojina race 7. The rate of explanation on the phenotype of related marker ranged from 2.00% to 15.47%. 20 positive alleles were revealed, among which Satt549-263(18.88) was the largest positive allele in the phenotypic effect value of C. sojina, and ‘Hefeng 29’ was the carrier material. The second one was Satt372-291(17.92), and the carrier material was ‘Hefeng 34’. The information of elite alleles and carrier materials could provide evidence for cross design for pyramiding elite alleles and marker-assisted selection in breeding of varieties resistant to C. sojina.

Key words: soybean, Cercospera sojina, Race 7, elite alleles, linkage disequilibrium

CLC Number:

S565.1

Jiang Hongjiao, Zhao Huiyan, Lin Chunyu, Liang Xiaoyu, Ma Shumei, Wang Yang. Soybean Variety Resource Resistant to Cercospora sojina Race 7: Mining of Elite Alleles[J]. Chinese Agricultural Science Bulletin, 2020, 36(31): 97-102.

Figures/Tables 6

References 28

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LD成对位点数	D'值次数分布(P<0.01)				D'平均值
LD成对位点数	0.1~0.3	0.4~0.5	0.6~0.7	0.8~1	D'平均值
1815	1206	416	132	61
12.2%	66.45%	22.92%	7.27%	3.36%	0.34

LD成对位点数	D'值次数分布(P<0.01)				D'平均值
LD成对位点数	0.1~0.3	0.4~0.5	0.6~0.7	0.8~1	D'平均值
1815	1206	416	132	61
12.2%	66.45%	22.92%	7.27%	3.36%	0.34

位点间遗传距离	LD成对位点数	总成对位点数	成对位点间LD频率/%	D'平均值
<1 cM	10	10	100	0.49
1~10 cM	28	71	39.44	0.41
11~20 cM	34	99	34.34	0.36
>20 cM	51	448	11.38	0.29
总计	123	628	19.59	0.32

位点间遗传距离	LD成对位点数	总成对位点数	成对位点间LD频率/%	D'平均值
<1 cM	10	10	100	0.49
1~10 cM	28	71	39.44	0.41
11~20 cM	34	99	34.34	0.36
>20 cM	51	448	11.38	0.29
总计	123	628	19.59	0.32

标记	染色体	物理距离	GLM		MLM
标记	染色体	物理距离	P值	贡献率/%	P值	贡献率/%
Sat-280	3	34710412	0.0272	2.45	0.018	2.86
Satt549	3	39360400	0.0000	15.47	0.0001	14.98
Satt454	5	25287731	0.0312	2.33	0.024	2.6
Satt200	5	40716815	0.0212	5.7	0.0428	5.05
Satt422	6	7227638	0.0232	5.72	0.0303	5.61
Sat-378	8	23605177	-	-	0.045	6.03
Satt247	9	9265135	0.0193	10.66	-	-
Satt260	9	39730973	0.0491	3	0.0288	3.63
Satt478	10	38560136	0.0034	4.6	0.0044	4.75
Sat-272	11	2710546	0.021	5.22	-	-
Satt509	11	6206957	0.0403	4.96	0.0343	5.33
Sat-214	12	14359	0.026	4.59	0.0173	5.22
Satt302	12	35108089	0.0189	2.77	0.0197	2.82
Satt212	15	5221921	-	-	0.0421	3.23
Satt685	15	48273606	0.0467	2	-	-
Sat-366	16	30036787	0.0138	3.08	0.0163	2.97
Satt372	17	7811367	0.0052	7.23	0.0074	7.23
Satt587	20	3738340	0.0425	3.19	-	-