Abstract:

The contamination of culture medium in tissue culture has always been a technical blind area in the field. The purpose of this experiment is to find a more efficient plant tissue culture system to inhibit the pollution of miscellaneous bacteria. By adding different concentrations and combinations of cefomycin, rifampicin, penicillin and other antibiotics and chemical drugs to MS medium for antibacterial screening, the antibacterial medium formula with cefomycin, chlorothalonil and mancozeb was determined. After that, the growth and contamination of Ipomoea batatas, Nicotiana tabacum and Arabidopsis thaliana on the medium were evaluated, and a new anti-contamination method of highly effective medium was obtained, namely liquid coating. Before explants were transplanted onto the medium with 25 mg/L cephalosporin and 30 mg/L 1:1 mixture of mancozeb and chlorothalonil, infiltrated and coated them with 100 mg/L carbendazim solution or the mixture of mancozeb and chlorothalonil at 1:1. By doing so, the risk of contamination risk of tissue culture materials would be significantly reduced with the growth of explants except callus being slightly affected. In general, the use of this method has less damage on the explant, and reference significance for rescuing contaminated tissue culture materials and the laboratory preservation of field germplasm materials.

Key words: plant tissue culture, anti-contamination formula with high efficiency, liquid coating, chlorothalonil, mancozeb, cephalosporin