Effect of IBA and NAA on Root Development of Sedum plumbizincicola Under Different pH

doi:10.11924/j.issn.1000-6850.casb20200200103

Abstract

Abstract:

To solve the negative impacts of the acidic environment on rooting of Sedum plumbizincicola cutting seedlings, a sand culture experiment was conducted in artificial climate chamber. In this experiment, both the influence of different pH conditions on rooting and plant growth of cutting seedlings and the effects of different concentrations of IBA and NAA on the rooting of cutting seedlings were studied. The results showed that, although cutting seedlings could grow normally, they did not grow well at pH 5.5. At pH≤5.0, the root system was underdeveloped, the stems were shorter and the leaves were smaller, eventually resulting in less than 40% of the normal biomass (pH 6.5), and the lower the pH, the faster the plant would die. Soaking the cutting seedlings with 0.5-10.0 mg/L IBA and NAA could significantly improve the survival rate through shortening rooting time and increasing rooting number and length. This research suggests that culture medium with pH≤5.0 is harmful to Sedum plumbizincicola cutting seedlings’ growth. Soaking the base of Sedum plumbizincicola cutting seedlings with 2.0-3.0 mg/L IBA and 1.0-2.0 mg/L NAA before cuttage can shorten the rooting time, increase the number and the length of the root system, and improve the survival rate.

Key words: Sedum plumbizincicola, cutting seedlings, pH, rooting, IBA, NAA

CLC Number:

S318

He Aiguo, Dai Yanjiao, Hu Zhixin, Wu Jiamei, Chen Jin. Effect of IBA and NAA on Root Development of Sedum plumbizincicola Under Different pH[J]. Chinese Agricultural Science Bulletin, 2020, 36(22): 49-54.

Figures/Tables 7

References 25

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pH	株高/cm	单株叶数/片	根长/cm	根数/条	生物量/(g/株)
4.0	7.13±0.42cC	21±2cC	0.17±0.06cC	2±0.58dD	0.0323±0.003dD
4.5	7.78±0.75cBC	21±2cC	0.20±0.10cC	5±2.59dD	0.0646±0.009cC
5.0	8.92±0.65bB	24±2bB	0.48±0.19cC	8±3.01dD	0.0624±0.007cCD
5.5	12.15±0.75aA	26±2bAB	1.72±0.34bB	18±4.34cC	0.1223±0.021bB
6.0	12.78±0.64aA	29±2aA	2.83±0.27aA	41±5.75bB	0.1495±0.017aAB
6.5	12.00±0.43aA	29±1aA	2.93±0.30aA	49±5.54aA	0.1648±0.006aA

pH	株高/cm	单株叶数/片	根长/cm	根数/条	生物量/(g/株)
4.0	7.13±0.42cC	21±2cC	0.17±0.06cC	2±0.58dD	0.0323±0.003dD
4.5	7.78±0.75cBC	21±2cC	0.20±0.10cC	5±2.59dD	0.0646±0.009cC
5.0	8.92±0.65bB	24±2bB	0.48±0.19cC	8±3.01dD	0.0624±0.007cCD
5.5	12.15±0.75aA	26±2bAB	1.72±0.34bB	18±4.34cC	0.1223±0.021bB
6.0	12.78±0.64aA	29±2aA	2.83±0.27aA	41±5.75bB	0.1495±0.017aAB
6.5	12.00±0.43aA	29±1aA	2.93±0.30aA	49±5.54aA	0.1648±0.006aA

pH	叶绿素a	叶绿素b	类胡萝卜素	叶绿素a+b
pH 4.0	1.80±0.36aA	0.55±0.12aA	0.40±0.08aA	2.35±0.48aA
pH 4.5	1.82±0.05aA	0.54±0.02aA	0.43±0.02aA	2.36±0.07aA
pH 5.0	1.13±0.08bB	0.32±0.03bB	0.26±0.02bB	1.45±0.11bB
pH 5.5	0.68±0.03cC	0.20±0.00cB	0.15±0.01cC	0.88±0.04cC
pH 6.0	0.62±0.05cC	0.18±0.01cB	0.13±0.02cC	0.80±0.07cC
pH 6.5	0.79±0.06cBC	0.27±0.05bcB	0.17±0.02cC	1.06±0.08cBC

pH	叶绿素a	叶绿素b	类胡萝卜素	叶绿素a+b
pH 4.0	1.80±0.36aA	0.55±0.12aA	0.40±0.08aA	2.35±0.48aA
pH 4.5	1.82±0.05aA	0.54±0.02aA	0.43±0.02aA	2.36±0.07aA
pH 5.0	1.13±0.08bB	0.32±0.03bB	0.26±0.02bB	1.45±0.11bB
pH 5.5	0.68±0.03cC	0.20±0.00cB	0.15±0.01cC	0.88±0.04cC
pH 6.0	0.62±0.05cC	0.18±0.01cB	0.13±0.02cC	0.80±0.07cC
pH 6.5	0.79±0.06cBC	0.27±0.05bcB	0.17±0.02cC	1.06±0.08cBC

激素	环境	0.0 mg/L	0.5 mg/L	1.0 mg/L	1.5 mg/L	2.0 mg/L	2.5 mg/L	3.0 mg/L	4.0 mg/L	6.0 mg/L	8.0 mg/L	10.0 mg/L
IBA	阴干	86.40bB	88.91aA	90.18aA	94.60aA	94.73aA	91.77aA	95.71aA	93.98aA	92.33aA	92.32aA	93.35aA
IBA	不阴干	79.35bB	84.18aA	89.55aA	90.90aA	94.27aA	93.26aA	92.92aA	93.59aA	91.24aA	91.57aA	90.23aA
NAA	阴干	86.79bB	93.72aA	92.40aA	93.39aA	94.71aA	92.73aA	94.38aA	93.39aA	94.05aA	93.72aA	90.42aA
NAA	不阴干	79.85bB	91.80aA	93.09aA	94.51aA	91.73aA	93.10aA	94.48aA	94.45aA	93.83aA	91.43aA	94.17aA