Salt Tolerance Function of Transcription Factor BvM14-GAI

doi:10.11924/j.issn.1000-6850.casb2021-0696

Abstract

Abstract:

Transcription factors play an important role in the process of plant response to adversity stress. GAI protein is an important member of the plant transcription factor family. About this protein, the research mainly focuses on the field of light response mechanism, while the protein response to salt tolerance mechanism is less reported. The full-length cDNA of BvM14-GAI gene, which is up-regulated in the transcriptome of the sugar beet M14 line under salt stress, is obtained in the early stage of the laboratory. This study aims to clarify the function of this gene in salt stress. By constructing the plant expression vector of BvM14-GAI gene, it is transformed into the Arabidopsis wild-type and GAI gene mutant plants by Agrobacterium-mediated inflorescence impregnation, then the phenotype and the indexes of the physiology and biochemistry of heterologous expression and heterologous complementary Arabidopsis plants under 150 mmol/L NaCl stress are detected. When treated with 0 mol/L NaCl, the root length, fresh weight and dry weight of heterologous expression and heterologous complementary Arabidopsis plants are significantly lower than those of the control group, indicating that BvM14-GAI gene is a negative growth regulator. After 150 mmol/L NaCl stress treatment, there is no significant difference in root length, fresh weight, dry weight and K ⁺/Na⁺ of the transgenic plants, but the contents of betaine, the enzyme activities of SOD and POD of the transgenic plants increase significantly, indicating that the transcription factor BvM14-GAI could improve the salt tolerance function of heterologous expression and heterologous complementation Arabidopsis plants by enhancing osmotic regulation and antioxidant enzyme systems. The study not only expands the function of plant GAI gene in response to abiotic stress, but also has research value for elucidating the salt-tolerant molecular mechanism of sugar beet M14 line and cultivating salt-tolerant crop lines.

Key words: sugar beet M14 line, BvM14-GAI gene, salt stress, transcription factor, antioxidant enzyme

CLC Number:

Q785

Ma Huimin, Sun Peilin, Ma Chunquan. Salt Tolerance Function of Transcription Factor BvM14-GAI[J]. Chinese Agricultural Science Bulletin, 2021, 37(34): 34-42.

Figures/Tables 9

References 30

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