Study on Dynamic Changes of AVR-Pia of Magnaporthe grisea in Southwestern Hubei

doi:10.11924/j.issn.1000-6850.casb2021-1080

Abstract

Abstract:

In order to understand the distribution and dynamic changes of AVR-Pia of Magnaporthe grisea in southwestern Hubei, 100 specific rice varieties were simultaneously planted in 9 locations in southwestern Hubei from 2017 to 2020, and the susceptible rice straws were collected to isolate monospore. We used the rice monogenic line IRBLa-A for pathogenicity identification, and designed specific primers for the avirulent gene AVR-Pia for PCR amplifying and sequence analyzing. The results showed that among the 661 isolates of Magnaporthe grisea isolated from 2017 to 2020, 49 isolates (7.4%) contained avirulent gene AVR-Pia. The frequencies of AVR-Pia were different significantly in different years and locations. From 2017 to 2020, the frequency of AVR-Pia was 7.5%, 16.0%, 3.1% and 5.0%, respectively. The location with the highest frequency of AVR-Pia was Yesanguan (15.8%), followed by Baiyang and Laifeng (both 14.6%), and no AVR-Pia was observed in Xianfeng, Jianshi and Taiping. The monogenic line IRBLa-A with Pia was resistant to 91 of the 661 isolates (13.8%). There were 49 isolates with 463 bp specific band of AVR-Pia, and no mutation was found in the CDS region through sequence analysis, but a base mutation G/T occurred at the 109 bp upstream of the start codon. Whether this point mution was related to expression needs further study. These results indicate that the avirulent gene AVR-Pia is less in southwestern Hubei, and the resistance gene Pia is not suitable for selective breeding as a major disease resistance gene in this region.

Key words: Magnaporthe grisea, AVR-Pia, dynamic changes, southwestern Hubei, variation mechanism

CLC Number:

S182

HAN Xuchen, DONG Yan, HAN Haojie, TAN Yanping, LIU Xuequn, LIU Xinqiong, XU Xin, LI Kai, WANG Chuntai. Study on Dynamic Changes of AVR-Pia of Magnaporthe grisea in Southwestern Hubei[J]. Chinese Agricultural Science Bulletin, 2022, 38(21): 112-121.

Figures/Tables 13

References 26

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序号	水稻品种	序号	水稻品种	序号	水稻品种	序号	水稻品种
1	IRBLA-A	26	草笛(Pik, Pish)	51	Pi20	76	杨优1号
2	IRBLA-C	27	梅雨明(Pikm)	52	ZD11	77	黔香优302
3	IRBLI-F5 (Pii)	28	福锦(PiZ, Pish)	53	ZD12	78	T优1655
4	IRBLks-F5	29	K1 (Pita)	54	ZD16	79	C两优755
5	IRBLKS-F5	30	Pi4号 (Pita2)	55	YTB	80	深优9516
6	IRBLK-KA	31	砦1号 (Pizt)	56	9311	81	全优2689
7	IRBLKP-K60(Pikp）	32	K60	57	kahei	82	宜香优62
8	IRBLKH-K3(Pikh）	33	BL1	58	骏优522	83	谷优92
9	IRBLZ-FU (Piz）	34	K59	59	丰城优质米	84	中9优恩66
10	IRBLZT-T(Pizt）	35	IRBLKM-TS	60	华占	85	宜香107
11	IRBLTA-CT2	36	IRBLTA2-RE	61	418B	86	中9优591
12	IRBLTA-CP1	37	特特普	62	R527	87	楚梗28
13	IRBLB-B(Pib)	38	珍龙13	63	骏1B	88	T优583
14	IRBLT-K59(Pit)	39	四丰43	64	R964	89	山珍
15	IRBLSH-S(Pish)	40	东农363	65	R522	90	ZJ1
16	IRBLSH-B	41	关东51	66	R172	91	ZJ2
17	F80-1	42	合江18	67	骏优172	92	ZJ3
18	F98-7	43	丽江	68	荣优华占	93	ZJ4
19	F124-1	44	IRBL1-CL (Pi1)	69	T优191	94	ZJ5
20	F128-1(Pita2)	45	IRBLZ5-CA (Piz5)	70	日本晴	95	ZJ6
22	F145-2	47	C104PKt	72	两优6206	97	ZJ8
23	新2号(Pish)	48	IRBL5-M	73	C两优651	98	ZJ9
24	爱知旭(Pia)	49	IRBL7-M	74	渝香203	99	ZJ10
25	藤坂5号(Pii)	50	IRBL9-W (Pi9)	75	陆267 两优267	100	华优8830

序号	水稻品种	序号	水稻品种	序号	水稻品种	序号	水稻品种
1	IRBLA-A	26	草笛(Pik, Pish)	51	Pi20	76	杨优1号
2	IRBLA-C	27	梅雨明(Pikm)	52	ZD11	77	黔香优302
3	IRBLI-F5 (Pii)	28	福锦(PiZ, Pish)	53	ZD12	78	T优1655
4	IRBLks-F5	29	K1 (Pita)	54	ZD16	79	C两优755
5	IRBLKS-F5	30	Pi4号 (Pita2)	55	YTB	80	深优9516
6	IRBLK-KA	31	砦1号 (Pizt)	56	9311	81	全优2689
7	IRBLKP-K60(Pikp）	32	K60	57	kahei	82	宜香优62
8	IRBLKH-K3(Pikh）	33	BL1	58	骏优522	83	谷优92
9	IRBLZ-FU (Piz）	34	K59	59	丰城优质米	84	中9优恩66
10	IRBLZT-T(Pizt）	35	IRBLKM-TS	60	华占	85	宜香107
11	IRBLTA-CT2	36	IRBLTA2-RE	61	418B	86	中9优591
12	IRBLTA-CP1	37	特特普	62	R527	87	楚梗28
13	IRBLB-B(Pib)	38	珍龙13	63	骏1B	88	T优583
14	IRBLT-K59(Pit)	39	四丰43	64	R964	89	山珍
15	IRBLSH-S(Pish)	40	东农363	65	R522	90	ZJ1
16	IRBLSH-B	41	关东51	66	R172	91	ZJ2
17	F80-1	42	合江18	67	骏优172	92	ZJ3
18	F98-7	43	丽江	68	荣优华占	93	ZJ4
19	F124-1	44	IRBL1-CL (Pi1)	69	T优191	94	ZJ5
20	F128-1(Pita2)	45	IRBLZ5-CA (Piz5)	70	日本晴	95	ZJ6
22	F145-2	47	C104PKt	72	两优6206	97	ZJ8
23	新2号(Pish)	48	IRBL5-M	73	C两优651	98	ZJ9
24	爱知旭(Pia)	49	IRBL7-M	74	渝香203	99	ZJ10
25	藤坂5号(Pii)	50	IRBL9-W (Pi9)	75	陆267 两优267	100	华优8830

引物名称	引物序列	目的片段长度/bp	NCBI GenBank number
AVR-Pia F1	TTATTGCTACCACCTTCCTC	463	AB498873.1
AVR-Pia R1	GTAGTAACTGAGTTGGCGTT	463
AVR-Pia F2	TCAGAGAAACGGACTTGGAGG	993
AVR-Pia R2	GTAATAGTCGCTGTAGTGCCG	993

引物名称	引物序列	目的片段长度/bp	NCBI GenBank number
AVR-Pia F1	TTATTGCTACCACCTTCCTC	463	AB498873.1
AVR-Pia R1	GTAGTAACTGAGTTGGCGTT	463
AVR-Pia F2	TCAGAGAAACGGACTTGGAGG	993
AVR-Pia R2	GTAATAGTCGCTGTAGTGCCG	993

地区	2017年			2018年
地区	总数	检出数	比例/%	总数	检出数	比例/%
咸丰	0	0	0	31	0	0
宣恩	17	0	0	18	0	0
柏杨	31	7	22.6	49	11	22.4
建始	27	0	0	3	0	0
太平	6	0	0	0	0	0
来凤	7	1	14.3	13	7	53.8
忠路	0	0	0	0	0	0
贺家坪	18	0	0	10	2	20.0
野三关	0	0	0	10	3	30.0
总计	106	8	7.5	134	23	16.0
地区	2019年			2020年
地区	总数	检出数	比例/%	总数	检出数	比例/%
咸丰	0	0	0	15	0	0
宣恩	11	0	0	69	7	10.1
柏杨	15	0	0	69	6	8.7
建始	55	0	0	1	0	0
太平	24	0	0	23	0	0
来凤	47	5	10.6	22	0	0
忠路	0	0	0	24	0	0
贺家坪	0	0	0	37	0	0
野三关	9	0	0	0	0	0
总计	161	5	3.1	260	13	5.0