Study on the Preparation and Purification Process of Sapogenin from Gleditsia sinensis

doi:10.11924/j.issn.1000-6850.casb2021-1088

Abstract

Abstract:

To optimize the preparation process of sapogenin from Gleditsia sinensis saponin by acid hydrolysis, the effects of acid concentration, acidolysis time, acidolysis temperature, material-to-liquid ratio and acid types on the conversion of saponin into sapogenin by acid hydrolysis were investigated. The results showed that the optimum conditions were as follows: hydrochloric acid concentration of 4 mol/L, hydrolysis time of 4 h, hydrolysis temperature of 80℃, solid-liquid ratio of 1:30 (m:v), and the content of Gleditsia sinensis sapogenin product was about 65%. The sapogenin after acid hydrolysis was purified by organic solvent extraction and recrystallization. The best extraction agent was trichloroethylene, the solid-liquid ratio was 1:20 (m:v), and extraction time did not change much within 90 minutes. After one extraction and recrystallization with 60% ethanol, the purity of total sapogenin could reach 76.31%.

Key words: saponin of Gleditsia sinensis, sapogenin, acidolysis, organic solvent extraction, recrystallization

CLC Number:

Q819

LIU Yanping, WANG Ying, ZHANG Jing, FAN Dingchen, LIU Jiajia, ZHAO Junjie, XUE Gang. Study on the Preparation and Purification Process of Sapogenin from Gleditsia sinensis[J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 143-149.

Figures/Tables 10

References 25

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酸种类	浓度/(mol/L)	皂荚皂苷元a/%	皂荚皂苷元b/%	皂苷元总量/%
硫酸	1	0.000	0.000	0.000
	1.5	29.952	0.000	29.952
	2	40.550	0.000	40.550
	2.5	41.304	0.000	41.304
	3	41.235	5.309	46.544
盐酸	2	50.149	14.443	64.592
	3	50.457	20.202	70.659
	4	50.481	16.455	70.682
	5	52.046	27.764	79.810
	6	42.944	25.291	68.235

酸种类	浓度/(mol/L)	皂荚皂苷元a/%	皂荚皂苷元b/%	皂苷元总量/%
硫酸	1	0.000	0.000	0.000
	1.5	29.952	0.000	29.952
	2	40.550	0.000	40.550
	2.5	41.304	0.000	41.304
	3	41.235	5.309	46.544
盐酸	2	50.149	14.443	64.592
	3	50.457	20.202	70.659
	4	50.481	16.455	70.682
	5	52.046	27.764	79.810
	6	42.944	25.291	68.235

酸种类	酸解时间/h	皂荚皂苷元a/%	皂荚皂苷元b/%	皂苷元总量/%
硫酸	3	0.000	0.000	0.000
	4	42.996	24.036	67.032
	5	48.002	22.959	70.961
	6	43.902	25.124	69.026
盐酸	3	45.935	27.980	73.915
	4	44.027	26.844	70.871
	5	45.361	24.469	69.830
	6	49.644	22.220	71.864

酸种类	酸解时间/h	皂荚皂苷元a/%	皂荚皂苷元b/%	皂苷元总量/%
硫酸	3	0.000	0.000	0.000
	4	42.996	24.036	67.032
	5	48.002	22.959	70.961
	6	43.902	25.124	69.026
盐酸	3	45.935	27.980	73.915
	4	44.027	26.844	70.871
	5	45.361	24.469	69.830
	6	49.644	22.220	71.864

酸种类	酸解温度/℃	皂荚皂苷元a/%	皂荚皂苷元b/%	皂苷元总量/%
硫酸	80	49.283	23.862	73.145
	85	43.342	12.793	56.135
	90	33.935	17.450	51.385
	95	41.646	19.828	61.474
盐酸	80	37.300	17.986	55.286
	85	39.397	16.067	55.464
	90	40.873	24.267	65.140
	95	46.331	23.442	69.773