The Rhizosphere Bacterial Community of Gastrodia elata Under Continuous Cropping in Yiliang County, Yunnan Province

doi:10.11924/j.issn.1000-6850.casb2022-0567

Abstract

Abstract:

In the development of Gastrodia elata industry, the problem of continuous cropping obstacle has gradually emerged, and the balance of microflora in the rhizosphere of Gastrodia elata has been damaged, which may be one of the causes of continuous cropping obstacles. To explore the effects of different numbers of continuous cropping on the structure and diversity of bacterial community in Gastrodia elata rhizosphere soil, the soil samples were collected from Yiliang County of Zhaotong City in Yunnan Province, including unplanted soil (cha001), the first crop soil (cha002) and the second crop soil (cha003), to analyze soil bacterial community by the high-throughput sequencing technology. The results showed that a total of 1245904 effective bacterial sequences and 2068 OTU were obtained, in which the OTU within the second crop soil (cha003) were the richest (1746 OTUs), and the bacterial community abundance (ACE index 1619.98, Chao1 index 1643.94) and diversity (Shannon index 8.82) were the highest. According to the diversity analysis, ACE index, Chao1 index and Shannon index increased with the continuous cropping period, while the Simpson index had no significant difference (P<0.05). Three phyla of Proteobacteria, Chloroflexi and Acidobacteria were dominant and the abundance of Proteobacteria and Acidobacteria increased with the continuous cropping period, while Chloroflexi showed a decreasing trend. The dominant classes were Acidobacteria, Alphaproteobacteria and Ktedonobacteria, the dominant orders were Acidobactertales, Rhizobiales, Solibacterales and Ktedonobacterales, and the dominant genera were uncultured_bacterium_o_Acidobacteriales, uncultured_bacterium_c_AD3 and Bryobacter. The continuous cropping obstacles could change the abundance and diversity of bacteria community of rhizosphere and destroy the original rhizosphere microecological balance, thus reducing the plant growth, increase the risk of diseases and pests, decrease the yield and benefit of Gastrodia elata production and have an impact on farmers' income.

Key words: Gastrodia elata, continuous cropping, rhizosphere soil, bacteria, high-throughput sequencing

CAI Liqiong, CHEN Rui, YANG Deqiang, ZHAO Changlin. The Rhizosphere Bacterial Community of Gastrodia elata Under Continuous Cropping in Yiliang County, Yunnan Province[J]. Chinese Agricultural Science Bulletin, 2023, 39(19): 80-87.

Figures/Tables 7

References 42

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处理	ACE指数	Chao1指数	辛普森指数	香农指数
cha001	1109.27±125.87a	1131.93±125.23a	0.99±0.01a	8.42±0.43a
cha002	1392.84±58.80b	1410.50±55.18b	0.99±0.01a	8.74±0.27a
cha003	1619.98±64.59c	1643.94±94.37c	0.99±0.01a	8.82±0.07a

处理	ACE指数	Chao1指数	辛普森指数	香农指数
cha001	1109.27±125.87a	1131.93±125.23a	0.99±0.01a	8.42±0.43a
cha002	1392.84±58.80b	1410.50±55.18b	0.99±0.01a	8.74±0.27a
cha003	1619.98±64.59c	1643.94±94.37c	0.99±0.01a	8.82±0.07a