Research Progress of Zinc Homeostasis Related Genes and Molecular Mechanism in Rice

doi:10.11924/j.issn.1000-6850.casb2022-0891

Abstract

Abstract:

Zinc (Zn) is an essential micronutrient for animals and plants. Zinc deficiency or excess can seriously affect the growth and development of rice. Maintaining zinc content in rice at a certain level is helpful to improve the yield and quality of rice, increase the zinc content in grain, and solve the current problem of zinc deficiency in human body to a certain extent. Therefore, it is important to understand Zn uptake, transport, distribution, and other molecular mechanisms regulating Zn homeostasis in rice. In this review, we briefly summarized the importance of zinc in plants, especially the ion transporters in rice and the molecular mechanisms. The roles of these ion transporters in the uptake of Zn from soil, the transport from root to shoot, and the distribution of Zn to various parts of rice were summarized. Some molecular mechanisms related to ion transporters were also summarized. This study provides reference for the mining of zinc homeostasis regulatory genes in rice, the study of molecular mechanism, and the creation of high zinc rice germplasm.

Key words: rice, zinc homeostasis, zinc transporter, molecular mechanism, research progress

LI Rongtian, LI Shuangyuyan, MENG Lijun, LIU Changhua, ZHAN Junhui. Research Progress of Zinc Homeostasis Related Genes and Molecular Mechanism in Rice[J]. Chinese Agricultural Science Bulletin, 2023, 39(32): 22-32.

Figures/Tables 4

References 98

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基因名称	基因序列号	表达部位	亚细胞定位	在酵母中锌转运活性	锌功能	参考文献
OsZIP1	LOC_Os01g74110	根、茎	内质网膜和质膜	否	吸收锌	[45]
OsZIP3	LOC_Os04g52310	节点	质膜	是	分配锌	[56]
OsZIP4	LOC_Os08g10630	根、茎	质膜	是	转运和分配锌	[53,57]
OsZIP5	LOC_Os05g39560	根	质膜	是	吸收锌	[49-50]
OsZIP7	LOC_Os05g10940	根、节点	质膜	是	转运和分配锌	[55,58]
OsZIP8	LOC_Os07g12890	根、茎	质膜	是	分配锌	[59]
OsZIP9	LOC_Os05g39540	根	质膜	是	吸收锌	[48,50]
OsIRT1	LOC_Os03g46470	根	质膜	是	吸收锌	[51,52]
OsHMA1	LOC_Os06g47550	根和茎	质体	-	-	[64-65]
OsHMA2	LOC_Os06g48720	根和节点	细胞质膜	-	转运锌	[66-67]
OsHMA3	LOC_Os07g12900	根和节点	液泡膜	-	转运锌	[68]
OsHMA9	LOC_Os06g45500	维管束和花药	细胞质膜	-	转运锌	[69]
OsNAS1	LOC_Os03g19427	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS2	LOC_Os03g19420	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS3	LOC_Os07g48980	叶	根的中柱鞘和伴胞，叶片的伴胞	-	-	[71-72]
OsNAAT1	LOC_Os02g20360	根和茎	原生木质部附近的伴胞和表皮细胞	-	-	[71-72]
OsMTP1	LOC_Os05g03780	叶片和茎	液泡膜	是	-	[74-75]
OsVIT1	LOC_Os04g38940	叶片	液泡膜	-	跨液泡膜运输锌	[75]
OsVIT2	LOC_Os09g23300	叶鞘	液泡膜	-	跨液泡膜运输锌	[76⇓-78]
OsZIFL12	LOC_Os12g03899	节1	液泡膜	-	分配锌	[81-82]

基因名称	基因序列号	表达部位	亚细胞定位	在酵母中锌转运活性	锌功能	参考文献
OsZIP1	LOC_Os01g74110	根、茎	内质网膜和质膜	否	吸收锌	[45]
OsZIP3	LOC_Os04g52310	节点	质膜	是	分配锌	[56]
OsZIP4	LOC_Os08g10630	根、茎	质膜	是	转运和分配锌	[53,57]
OsZIP5	LOC_Os05g39560	根	质膜	是	吸收锌	[49-50]
OsZIP7	LOC_Os05g10940	根、节点	质膜	是	转运和分配锌	[55,58]
OsZIP8	LOC_Os07g12890	根、茎	质膜	是	分配锌	[59]
OsZIP9	LOC_Os05g39540	根	质膜	是	吸收锌	[48,50]
OsIRT1	LOC_Os03g46470	根	质膜	是	吸收锌	[51,52]
OsHMA1	LOC_Os06g47550	根和茎	质体	-	-	[64-65]
OsHMA2	LOC_Os06g48720	根和节点	细胞质膜	-	转运锌	[66-67]
OsHMA3	LOC_Os07g12900	根和节点	液泡膜	-	转运锌	[68]
OsHMA9	LOC_Os06g45500	维管束和花药	细胞质膜	-	转运锌	[69]
OsNAS1	LOC_Os03g19427	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS2	LOC_Os03g19420	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS3	LOC_Os07g48980	叶	根的中柱鞘和伴胞，叶片的伴胞	-	-	[71-72]
OsNAAT1	LOC_Os02g20360	根和茎	原生木质部附近的伴胞和表皮细胞	-	-	[71-72]
OsMTP1	LOC_Os05g03780	叶片和茎	液泡膜	是	-	[74-75]
OsVIT1	LOC_Os04g38940	叶片	液泡膜	-	跨液泡膜运输锌	[75]
OsVIT2	LOC_Os09g23300	叶鞘	液泡膜	-	跨液泡膜运输锌	[76⇓-78]
OsZIFL12	LOC_Os12g03899	节1	液泡膜	-	分配锌	[81-82]

基因名称	基因序列号	ZDRE元件数量	ZDRE元件	元件位置^*	碱基替换数量
OsZIP3	LOC_Os04g52310	1	GTGTCGCCTT	-1388	2
OsZIP4	LOC_Os08g10630	1	ACATCGACAC	-568	2
OsZIP5	LOC_Os05g39560	2	ATGTCGACAC	-790	0
OsZIP5	LOC_Os05g39560	2	GAATCGACAT	-480	2
OsZIP7	LOC_Os05g10940	2	ATGTCGACAC	-631	0
OsZIP7	LOC_Os05g10940	2	GTGACGACAT	-288	1
OsZIP8	LOC_Os07g12890	3	ATGTCAACAC	-283	1
			GTGTCGTCAT	-260	1
			GTGTCGTCAC	-154	1
OsZIP9	LOC_Os05g39540	2	ATGGCGACAT	-2027	1
OsZIP9	LOC_Os05g39540	2	ATGTCGACAC	-1772	0
OsZIP10	LOC_Os06g37010	3	GTGTCGACAC	-704	0
			ATGACGACAT	-485	1
			ATGACGACAT	-455	1
OsHMA1	LOC_Os06g47550	4	ATGTCGAACC	-570	2
			GTGTCGGAGC	-693	3
			GGTTCGACAC	-2753	2
			GTGACGACAC	-2764	1
OsHMA3	LOC_Os07g12900	1	ATTGCGACAT	-1354	2
OsHMA9	LOC_Os06g45500	1	GTGTCGTCTT	-1085	2
OsNAS1	LOC_Os03g19427	2	GTGTCGCTCC	-1887	3
OsNAS1	LOC_Os03g19427	2	ATACCGACAC	-2404	2
OsNAS2	LOC_Os03g19420	3	ATACCGACAC	-720	2
			GTGTCTAAAT	-486	2
			GTGTCGCTCC	-1227	3
OsNAS3	LOC_Os07g48980	1	ATGCTGACAT	-1767	2
OsMTP1	LOC_Os05g03780	1	AACACGACAT	-1078	3
OsVIT1	LOC_Os04g38940	3	ATTTGGACAT	-1211	2
			GTGTCGGTCT	-1847	3
			GTAGAGACAT	-1902	3
OsVIT2	LOC_Os09g23300	1	GTGCAGACAT	-2407	2
OsZIFL12	LOC_Os12g03899	2	GTGTCGCCGT	-2426	2
OsZIFL12	LOC_Os12g03899	2	AAACCGACAT	-2922	3
OsFRDL1	LOC_Os03g11734	1	GTGTCGTTGT	-1296	3

基因名称	基因序列号	ZDRE元件数量	ZDRE元件	元件位置^*	碱基替换数量
OsZIP3	LOC_Os04g52310	1	GTGTCGCCTT	-1388	2
OsZIP4	LOC_Os08g10630	1	ACATCGACAC	-568	2
OsZIP5	LOC_Os05g39560	2	ATGTCGACAC	-790	0
OsZIP5	LOC_Os05g39560	2	GAATCGACAT	-480	2
OsZIP7	LOC_Os05g10940	2	ATGTCGACAC	-631	0
OsZIP7	LOC_Os05g10940	2	GTGACGACAT	-288	1
OsZIP8	LOC_Os07g12890	3	ATGTCAACAC	-283	1
			GTGTCGTCAT	-260	1
			GTGTCGTCAC	-154	1
OsZIP9	LOC_Os05g39540	2	ATGGCGACAT	-2027	1
OsZIP9	LOC_Os05g39540	2	ATGTCGACAC	-1772	0
OsZIP10	LOC_Os06g37010	3	GTGTCGACAC	-704	0
			ATGACGACAT	-485	1
			ATGACGACAT	-455	1
OsHMA1	LOC_Os06g47550	4	ATGTCGAACC	-570	2
			GTGTCGGAGC	-693	3
			GGTTCGACAC	-2753	2
			GTGACGACAC	-2764	1
OsHMA3	LOC_Os07g12900	1	ATTGCGACAT	-1354	2
OsHMA9	LOC_Os06g45500	1	GTGTCGTCTT	-1085	2
OsNAS1	LOC_Os03g19427	2	GTGTCGCTCC	-1887	3
OsNAS1	LOC_Os03g19427	2	ATACCGACAC	-2404	2
OsNAS2	LOC_Os03g19420	3	ATACCGACAC	-720	2
			GTGTCTAAAT	-486	2
			GTGTCGCTCC	-1227	3
OsNAS3	LOC_Os07g48980	1	ATGCTGACAT	-1767	2
OsMTP1	LOC_Os05g03780	1	AACACGACAT	-1078	3
OsVIT1	LOC_Os04g38940	3	ATTTGGACAT	-1211	2
			GTGTCGGTCT	-1847	3
			GTAGAGACAT	-1902	3
OsVIT2	LOC_Os09g23300	1	GTGCAGACAT	-2407	2
OsZIFL12	LOC_Os12g03899	2	GTGTCGCCGT	-2426	2
OsZIFL12	LOC_Os12g03899	2	AAACCGACAT	-2922	3
OsFRDL1	LOC_Os03g11734	1	GTGTCGTTGT	-1296	3

基因名称	基因序列号	RR元件(AGATT)	基因名称	基因序列号	RR元件(AGATT)	基因名称	基因序列号	RR元件(AGATT)
OsZIP1	LOC_Os01g74110	2	OsZIP11	LOC_Os05g25194	6	OsHMA9	LOC_Os06g45500	5
OsZIP2	LOC_Os03g29850	5	OsZIP13	LOC_Os02g10230	7	OsNAS1	LOC_Os03g19427	3
OsZIP3	LOC_Os04g52310	8	OsZIP14	LOC_Os08g36420	2	OsNAS3	LOC_Os07g48980	5
OsZIP4	LOC_Os08g10630	3	OsZIP16	LOC_Os08g01030	3	OsVIT1	LOC_Os04g38940	1
OsZIP5	LOC_Os05g39560	4	OsIRT1	LOC_Os03g46470	3	OsVIT2	LOC_Os09g23300	7
OsZIP6	LOC_Os05g07210	3	OsIRT2	LOC_Os03g46454	4	OsNAAT1	LOC_Os02g20360	4
OsZIP7	LOC_Os05g10940	2	OsHMA1	LOC_Os06g47550	2	OsMTP1	LOC_Os05g03780	5
OsZIP8	LOC_Os07g12890	1	OsHMA2	LOC_Os06g48720	8	OsZIFL12	LOC_Os12g03899	4
OsZIP9	LOC_Os05g39540	8	OsHMA3	LOC_Os07g12900	5	OsFRDL1	LOC_Os03g11734	3
OsZIP10	LOC_Os06g37010	2