Anesthetic Effect of Eugenol on Juvenile Schizothorax chongi

doi:10.11924/j.issn.1000-6850.casb2023-0222

Abstract

Abstract:

To investigate the anesthetic effect of eugenol on junvenile Schizothorax chongi and provide theoretical basis for artificial breeding of Schizothorax chongi, we measured the time of anesthesia and recovery under different concentration, different anesthetic time and different exposure time by hydrostatic method. The results showed that a negative correlation between the eugenol concentration and anesthesia time was identified with y₁=286.63e^-0.0135^x, R²=0.9593; a positive correlation between the eugenol concentration and recovery time was found with y₂=101.54e^0.0134^x, R²=0.9913; the anesthetic time and the recovery time differed significantly with different concentration of eugenol (P<0.05); the recovery time was positively correlated with the anesthetic time (y=64.647x^0.2394, R²=0.9823) under concentration of eugenol was 40 mg/L; a significant negative correlation between recovery time and exposure time was identified with y=900.92x^-0.4078, R²=0.8961. The conclusion of this study was that eugenol concentration of 40-80 mg/L was the best in the production, transportation and sampling; the medicinal bath time of young fish should not exceed 600 s and the exposure time of juveniles were permitted no more than 1200 s.

Key words: eugenol, Schizothorax chongi, anesthesia effect, duration of exposure, duration of recovery

ZUO Pengxiang, JIN Fangpeng, LENG Yun, WANG Zhifei, WU Junjie, YU Hongman, DENG Yulin, ZU Xuepeng, LI Minghui, WANG Jianbo, ZHANG Xinghai. Anesthetic Effect of Eugenol on Juvenile Schizothorax chongi[J]. Chinese Agricultural Science Bulletin, 2024, 40(5): 159-164.

Figures/Tables 6

References 29

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处理浓度/(mg/L)	麻醉时间/s		复苏时间/s		复苏率/ %	复苏后72 h存活率/ %
处理浓度/(mg/L)	范围	均值	范围	均值	复苏率/ %	复苏后72 h存活率/ %
20	234~298	267.33±21.03^a	123~153	138.07±9.72^A	100	100
30	157~199	178.9±13.91^b	134~173	154.53±12.36^B	100	100
40	131~164	149.10±10.40^c	154~195	175.83±13.56^C	100	100
50	127~148	136.87±6.60^d	167~206	186.73±12.09^D	100	100
60	112~134	123.33±6.48^e	196~226	211.07±12.36^E	100	100
80	86~107	96.13±6.57^f	283~330	309.27±16.30^F	100	100
100	69~88	78.97±6.27^g	376~416	398.83±12.95^G	100	100
120	49~68	57.23±4.88^h	486~526	506.23±12.59^H	95	80

处理浓度/(mg/L)	麻醉时间/s		复苏时间/s		复苏率/ %	复苏后72 h存活率/ %
处理浓度/(mg/L)	范围	均值	范围	均值	复苏率/ %	复苏后72 h存活率/ %
20	234~298	267.33±21.03^a	123~153	138.07±9.72^A	100	100
30	157~199	178.9±13.91^b	134~173	154.53±12.36^B	100	100
40	131~164	149.10±10.40^c	154~195	175.83±13.56^C	100	100
50	127~148	136.87±6.60^d	167~206	186.73±12.09^D	100	100
60	112~134	123.33±6.48^e	196~226	211.07±12.36^E	100	100
80	86~107	96.13±6.57^f	283~330	309.27±16.30^F	100	100
100	69~88	78.97±6.27^g	376~416	398.83±12.95^G	100	100
120	49~68	57.23±4.88^h	486~526	506.23±12.59^H	95	80

药浴时间/s	复苏时间/s		复苏率/ %	复苏后72 h 存活率/%
药浴时间/s	范围	均值	复苏率/ %	复苏后72 h 存活率/%
300	202~291	248.3±26.47^a	100%	100%
420	220~319	275.10±34.86^b	100%	100%
600	282~326	304.87±14.16^c	100%	100%
900	310~368	338.77±17.19^d	100%	80%
1200	322~376	349.57±16.62^e	70%	60%
1500	336~391	365.33±17.74^f	50%	40%

药浴时间/s	复苏时间/s		复苏率/ %	复苏后72 h 存活率/%
药浴时间/s	范围	均值	复苏率/ %	复苏后72 h 存活率/%
300	202~291	248.3±26.47^a	100%	100%
420	220~319	275.10±34.86^b	100%	100%
600	282~326	304.87±14.16^c	100%	100%
900	310~368	338.77±17.19^d	100%	80%
1200	322~376	349.57±16.62^e	70%	60%
1500	336~391	365.33±17.74^f	50%	40%

暴露时间/s	复苏时间/s		复苏率/%	复苏后72 h 存活率/%
暴露时间/s	范围	均值	复苏率/%	复苏后72 h 存活率/%
120	94~118	106.87±7.92^a	100	100
300	83~102	93.50±6.18^b	100	100
480	72~90	81.60±6.49^c	100	100
660	63~82	72.87±6.74^d	100	100
840	54~73	63.43±5.61^e	100	100
1020	46~66	56.32±6.50^f	100	100
1200	44~60	52.26±5.53^g	100	100
1380	36~52	44.19±6.01^h	90	80
1560	31~51	42.03±5.66ⁱ	75	60
1800	27~41	35.19±4.57^j	60	50