Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice

doi:10.11924/j.issn.1000-6850.casb2023-0549

Abstract

Abstract:

The research aims to lay the foundation for map-based cloning of the leaf color related gene by analyzing the phenotypic characteristics of a rice yellow-green leaf mutant ygl-9108 and mapping the mutant locus. A yellow-green leaf mutant, named as ygl-9108, was selected as experimental material, and the gene was mapped by using the F₂ population derived from the cross between the ygl-9108 mutant and ‘Wushansimiao’. The results showed that the plant height, effective panicle number, panicle length, grain number per panicle, seed setting rate, grain width, 1000-grain weight, and grain weight per plant of the ygl-9108 mutant were all significantly reduced by 21.5%, 21.2%, 14.6%, 19.2%, 11.0%, 10.2%, 12.9%, and 52.2%, respectively, compared with those of the wild type. The observation by transmission electronic microscope (TEM) demonstrated that the number of chloroplasts in mesophyll cells of ygl-9108 plants was greatly reduced, and the lamellar structure of chloroplasts was abnormal. Genetic analysis revealed that the phenotype of mutant was controlled by a single recessive nuclear gene. Using a map-based cloning strategy, ygl-9108 was mapped to a 147 kb region on chromosome 11 between two InDel markers, 11Y39 and 11Y45. No gene related to leaf color has been reported previously in this interval, indicating that ygl-9108 is a new leaf color-related gene. This study lays a solid foundation for further research on gene cloning and function analysis of ygl-9108.

Key words: Oryza sativa L., yellow-green leaf mutants, ygl-9108, phenotypic identification, agronomic trait, gene mapping, transmission electron microscope, chloroplast, recessive nuclear gene, map-based cloning

LIU Xiaomin, SUN Zhiguang, CHI Ming, XING Yungao, XU Bo, LI Jingfang, LIU Yan, LU Baiguan, WANG Baoxiang, XU Dayong. Identification and Gene Mapping of A Novel Yellow-green Leaf Mutant ygl-9108 in Rice[J]. Chinese Agricultural Science Bulletin, 2024, 40(26): 22-29.

Figures/Tables 5

References 34

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