Influence Mechanisms of Pine Needle Extracts on Tea Plant Growth and Pest Control

doi:10.11924/j.issn.1000-6850.casb2023-0605

Abstract

Abstract:

In order to explore the effect of pine needle extracts on tea plant growth and pest control, tea trees treated with and without pine needle extracts were used as experiment materials, and the combined transcriptome and metabolome analysis was conducted to analyze the internal mechanism. The results showed that pine needle extracts could promote the growth of tea plants, with the increasing germination density, the relative content of chlorophyll in tea plant leaves, and the yield of tea without reducing the quality of tea. In this process, the expression of AP2/ERF transcription factors was suppressed, and ethylene signaling pathway played an important role of negative regulator. Besides, pine needle extracts treatments did not affect the community structure of arthropods of tea plants, and displayed a certain control effect on mites and tea green leafhoppers. The combined analysis of transcriptome and metabolome showed that the improvement of insect resistance of tea plant might be due to the activation of polyphenol and phospholipid metabolic pathways of tea plants, and the contents of corresponding metabolites were significantly increased. Thus, the antioxidant and free radical scavenging ability of tea plants were improved, so as to enhance the stress resistance of tea plants. In general, the results can provide a basis for the development and utilization of natural plant stress inducers.

Key words: pine needle extracts, Camellia sinensis, ethylene signaling pathway, polyphenols, antioxidant, pest management, transcriptomics, metabolomics, plant resistance inducers

ZHANG Man, LI Jianlong, ZHOU Bo, CHEN Yiyong, NONG Hongqiu, CUI Yingying, LIU Jiayu, TANG Jinchi. Influence Mechanisms of Pine Needle Extracts on Tea Plant Growth and Pest Control[J]. Chinese Agricultural Science Bulletin, 2024, 40(25): 103-112.

Figures/Tables 15

References 29

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处理	项目	外形(25%)	汤色(10%)	香气(25%)	滋味(30%)	叶底(10%)	总分
CK	评语	紧实略卷曲毫乌褐	红稍深，较明亮	较甜	甘醇，微涩	较匀，尚红	88.95±0.919a
CK	分值	88.5±0.707a	91.5±0.707a	90.0±0.001a	87.75±3.889a	88±1.414a	88.95±0.919a
PNEs	评语	紧实略卷曲毫乌褐	红稍深，较明亮	较甜	甘醇，微涩	较匀，尚红	88.80±0.989a
PNEs	分值	88.0±0.0001a	92±0.0001a	91.0±1.414a	87±4.242a	87±0.0001a	88.80±0.989a

处理	项目	外形(25%)	汤色(10%)	香气(25%)	滋味(30%)	叶底(10%)	总分
CK	评语	紧实略卷曲毫乌褐	红稍深，较明亮	较甜	甘醇，微涩	较匀，尚红	88.95±0.919a
CK	分值	88.5±0.707a	91.5±0.707a	90.0±0.001a	87.75±3.889a	88±1.414a	88.95±0.919a
PNEs	评语	紧实略卷曲毫乌褐	红稍深，较明亮	较甜	甘醇，微涩	较匀，尚红	88.80±0.989a
PNEs	分值	88.0±0.0001a	92±0.0001a	91.0±1.414a	87±4.242a	87±0.0001a	88.80±0.989a

处理	水分	水浸出物	茶多酚	游离氨基酸	咖啡碱
CK	3.29±0.045a	41.22±0.041a	8.37+0.044b	3.57+0.091a	3.91±0.045b
PNEs	3.36±0.066a	41.25±0.24a	9.38±0.12a	3.64±0.27a	3.61±0.035a

处理	水分	水浸出物	茶多酚	游离氨基酸	咖啡碱
CK	3.29±0.045a	41.22±0.041a	8.37+0.044b	3.57+0.091a	3.91±0.045b
PNEs	3.36±0.066a	41.25±0.24a	9.38±0.12a	3.64±0.27a	3.61±0.035a

序号	组分名称	相对含量		序号	组分名称	相对含量
序号	组分名称	CK	PNEs	序号	组分名称	CK	PNEs
1	β-芳樟醇	52.22	50.17	21	3-苯基呋喃	0.50	0.55
2	水杨酸甲酯	16.59	18.40	22	反-3-己烯醇	0.42	0.40
3	苯乙醛	4.90	5.38	23	香叶基丙酮	0.44	0.46
4	β-紫罗酮	2.81	2.80	24	橙花醇	0.40	0.38
5	苯甲醛	2.44	2.75	25	顺-茉莉酮	0.35	0.34
6	橙花叔醇	1.76	1.88	26	反-2-己烯醇	0.34	0.34
7	顺-己酸-3-己烯酯	1.64	1.50	27	柠檬烯	0.29	0.31
8	香叶醇	1.63	1.34	28	糠醛	0.27	0.26
9	2-己烯醛	1.52	1.42	29	环氧芳樟醇	0.26	0.27
10	二甲硫	1.42	1.47	30	正己醛	0.25	0.24
11	2-苯基-2-丁烯醛	1.27	1.20	31	3-甲基丁醛	0.25	0.23
12	苯乙醇	1.05	0.90	32	己酸己酯	0.24	0.25
13	氧化芳樟醇Ⅰ	0.94	0.84	33	反-2-反-4庚二烯醛	0.24	0.18
14	反-戊酸-2-己烯酯	0.78	0.79	34	顺-3-己烯酸-3-己烯酸酯	0.18	0.18
15	β-蒎烯	0.76	0.77	35	α-蒎烯	0.18	0.22
16	2-甲基丁醛	0.69	0.75	36	己醇	0.17	0.15
17	β-环柠檬醛	0.62	0.66	37	2-乙基呋喃	0.15	0.15
18	α-紫罗酮	0.61	0.53	38	反-2-戊烯醛	0.10	0.10
19	5-甲基-2-苯基2-己烯醛	0.60	0.71	39	1-辛烯-3-醇	0.10	0.10
20	反,反-2,4-己二烯醛	0.52	0.53	40	庚醛	0.10	0.10